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Gene Expression of Perilipin and Its Regulation in Pigs

Author: ZhangXiaoWei
Tutor: YangXiaoJing；ZhaoRuZuo
School: Nanjing Agricultural College
Course: Basic Veterinary Science
Keywords: Pig Fat metabolism Perilipin Glucocorticoids
CLC: S828
Type: Master's thesis
Year: 2009
Downloads: 22
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Abstract


This study was aimed to investigate expression of perilipin in adipose tissue of pigs and its regulation by maternal nutrition and glucocorticoids exposure in vitro. The adipose tissue was taken from weaned piglets which were born to sows fed with diets containing different protein levels and from pigs with different halothane genotype (HalNN, homeozygous wild type and Halnn, homozygous mutant), followed by quantification of mRNA expression of perilipin and other related regulating genes. Furthermore, stromal-vascular cells were isolated from porcine adipose tissue and treated with dexamthasone to elucidate the effects of glucocorticoid on lipid storage and gene expression of perilipin, as well as its potential regulation mechanism.1 Effect of maternal dietary protein restriction on fat deposition and perilipin mRNA expression in adipose tissue of weaning pigletsSows in control group were fed with diets containing 12%and 14%of protein during pregnancy and lactation respectively, whereas protein levels reduced by half in treated group. Piglets born to sows in both control and treated groups were sacrificed after weaning. Back fat thickness of weaned piglets was measured. Gene expression of perilipin and ADRP were determined with relative quantitative real time RT-PCR. The results showed that index of back fat thickness of piglets in treated group were significantly smaller than that in control group. Piglets in treated group exhibited significantly lower expression of perilipin mRNA (P< 0.05) than that in treated group. There was no significant difference in ADRP mRNA expression between control and treated groups.2 Expression of perilipin and lipolytic genes in adipose tissue of pigs with different halothane genotypesGene expression was determined with relative quantitative real time RT-PCR. Halnn pigs exhibited significantly higher perilipin mRNA expression (P< 0.05), but lower GR mRNA expression than that in HalNN pigs (P< 0.05). There were no significant differences between fat depots in perilipin or GR mRNA expression in both Halnn and HalNN pigs. Significant fat depot differences were found for 110-HSD1, FAS and HSL mRNA expression. However, gene expression of C/EBPβand PPARγexhibited no differences neither between halothane genotypes nor depots. These results suggest that subcutaneous and omental adipose tissue demonstrates significantly different expression patterns of some lipid metabolic genes in the pig. Furthermore, perilipin mRNA expression exhibits significant difference between HalNN and Halnn pig, and this difference may be related to GR.3 Effect of dexamthasone on lipid droplet deposition and related genes expression in primary cultured adipocytes of pigs and its regulating pathwaySV cells were separated from subcutaneous adipose tissue of weaned piglet. Cells were cultured to 80% confluence followed by treatments with 10 M dexamethasone with or without GR antagonist RU486. Triglyceride content in cultured adipocytes was significantly increased after dexamthasone treatment for 48 h compared with control group, and this effect can be blocked by RU486. Real time RT-PCR results showed that dexamethasone could significantly up-regulate gene expression of perilipin and PPARy whereas down-regulate C/EBPβmRNA expression. Yet GR mRNA expression in dexamethasone treated group did not change up to a significant level compared to control group. Taken together, conclusion can be made that glucocorticoids increase triglyceride content though up-regulating expression of perilipin and PPARy may be involved in this effect.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Livestock > Pig
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