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The Association between Androgen Deficiency, Obesity and Lipid Metabolism Genes in Males

Author: HeHua
Tutor: YuanWei
School: Fudan University
Course: Epidemiology and Biostatistics,
Keywords: PADAM Testosterone Testosterone secretion index BMI Waistline D-galactose Leydig cell Gene chip RT-PCR Perilipin
CLC: R589.2
Type: Master's thesis
Year: 2011
Downloads: 65
Quote: 0
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Abstract


Background With the rapid development of medical science, health care has improved continuously, the average life expectancy of the population worldwide generally extended the proportion of older people is rising, the world is moving into the era of aging. Therefore focus on the middle-aged male reproductive health, and to improve the quality of life, has become the the medically important topics of global concern. Modern medicine for middle-aged men with partial androgen deficiency (PADAM) factors and the understanding of the pathogenesis of a long, controversial process. Among them, the relationship between obesity and androgen levels has become a research focus in recent years. Studies have shown that there are varying degrees of androgen levels in male obese patients decreased, suggesting that there may be a certain relationship between obesity and androgen levels decline. Target epidemiological status of survey methods to explore the relationship between obesity and androgen levels caused by PADAM, and animal experiments to further explore the relationship between obesity-related lipid metabolism genes were differentially expressed with androgen deficiency . [Methods the epidemiological investigation part of this study is based on the current situation of the community population. PADAM prevalence of middle-aged men were eligible for inclusion, through the detection of clinical androgen, middle-aged men are overweight or obese, the occurrence of mild and severe abdominal obesity combined with physical examination, and basic demographic characteristics of the study through questionnaires general health, PADAM related factors. Explore the relationship between obesity and PADAM analysis, based on the control of potential confounding factors of age, education, work status, disease status and medication compliance, the Wilcoxon test was used measure of outcome indicators were analyzed using the card square test and logistic regression analysis the count indicator analysis. The method the the animal experimental part of this study using D-galactose injection to establish animal models of rats subacute aging androgen deficiency (PADAM), serum total testosterone (TT), superoxide dismutase (SOD), malondialdehyde (MDA) levels to determine whether the model was successful using discontinuous Percoll density gradient centrifugation and purified Leydig cells (Leydig), extracted rat Leydig cell total RNA after primary culture after 72h further gene chip screening obese fat Metabolism differentially expressed genes and semi-quantitative RT-PCR method results verify. [Results] The epidemiological investigation part of this study participate in the survey and fill out a complete, 980 cases involved in blood collection study 944 cases involved in the study of 980 cases of physical examination, physical examination complete, 968 cases; study androgen deficiency diseases The disease was 18.01%; the Univariate Logistic regression found that overweight and obese group, mild abdominal obesity group, severe abdominal obesity group suffering from male hormone deficiency dangerous are 2.72 times the corresponding normal or low group ( 95% CI :1.80-4 .11), 3.19-fold (95% CI :1.91-5 .32), 1.67-fold (95% CI :1.10-2 .53) and 3.06-fold (95% CI, :1.94-4 .82); Multivariate Logistic regression control with androgen deficiency disease related factors (age, education level, work status, disease and medication situation) found that overweight and obese group, mild abdominal obesity group, suffering from severe abdominal obesity group androgen deficiency dangerous are 2.51 times the corresponding normal or low group (95% CI :1.65-3 .81), 2.95-fold (95% CI, :1.74-5 .01) and 1.61-fold (95% CI, :1.05-2.47), 2.75 times (95% CI :1.72-4 .41). Part of the results of animal experiments in this study show that compared with the control group, androgen deficiency model group (low-dose group and high dose group) serum total testosterone (TT) concentrations, decreased serum superoxide dismutase (SOD) activity, malondialdehyde dialdehyde (MDA) content increased, the difference was statistically significant; purity of Leydig cells from an average of 94.5%; gene chip results showed that androgen deficiency model group compared with control group obesity related to lipid metabolism differentially expressed genes 5, are Perilipin, Fabp4, Mgl1, Mgl-1, Lipe; Finally, using semi-quantitative RT-PCR validation of microarray results significantly down-regulated gene expression found of Perilipin its two subtypes. [Conclusion] obesity may be associated with PADAM occurrence; obesity-related gene the Perilipin with androgen lack of lipid metabolism may be relevant at the RNA level.

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CLC: > Medicine, health > Internal Medicine > Endocrine diseases and metabolic diseases > Metabolic diseases > Lipodystrophy
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