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Transformation of Rice OsNPR1 for Pepper and the Construction of CaNPR1-RNAi Transgenic Pepper

Author: LuoHuan
Tutor: FengJiaXun
School: Guangxi University
Course: Biochemistry and Molecular Biology
Keywords: rice OsNPR1 gene pepper CaNPR1 gene RNAi transformation
CLC: Q943.2
Type: Master's thesis
Year: 2006
Downloads: 13
Quote: 0
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Abstract


The Arabidopsis thaliana NPR1 is a positive regulator in systemic acquired resistance(SAR). We cloned OsNPR1, an Arabidopsis thaliana NPR1 homologous gene in rice, and confirmed it played a positive role in rice disease resistance regulation as a key regulator. In order to investigate that overexpress monocotyledonous rice OsNPR1 gene in dicotyledonous pepper(Capsicum annuum) whether or not enhance the broad-spectrum disease resistance in pepper, in this work, a pepper expression vector of OsNPR1 gene was constructed. Pepper cultivar Tiandeng1 was transformed with OsNPR1 gene by Agrobacterium tumefaciens-medmted transformation. Eight kanamycin-resistant transgenic seedlings were obtained. It can provide the plant materials for next identity of disease resistance.The full-length cDNA of CaNPR1, an Arabidopsis thaliana NPR1 homologous gene in pepper was cloned. CaNPR1 and NPR1 share 62.9% homology at mRNA level and their encoded products have 49.7% identity and 65.7% similarity. In order to identify the function of CaNPR1 in SAR of pepper, an expression vector pCaNPRl-KNAi for silencing CaNPR1 in pepper was constructed. Pepper cultivar Guiyan5 was tansformed with pCaNPR1-RNAi by Agrobacterium tumefaciens-mediated transformation. Six kanamycin-resistant transgenic seedlings were obtained. PCR identity and Southern hybridization analysis confirmed that all the six regenerated seedlings were transgenic plants. This work can supply the materials for next functional identification of CaNPR1.

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CLC: > Biological Sciences > Botany > Plant Cell Genetics > Plant Genetic Engineering
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