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To study on a new industrialization pathway that some plant cells produce resveratrol (a kind of secondary metabolin), the grape cells were used as a research object in this paper. The foundation and optimization of a suspension culture system were acted as the aims. Based on some single factor tests and the orthogonal trials of four main factors, grape callus were induced, suspension cell system were preliminary erected and suspension culture medium and condition were optimized.The leaves, leafstalk, and stems from grapes were used as explants to induce calli and subculture in medium with different plant hormone combination; Te relaxed calli was used to erect suspension cell system, and then the resveratol content was determinated by using HPLC.The results were indicated that, the calli development rate from leaves inducement was lower(under 10%) than that from leafstalk(maximum 96.7%), and young stems(maximum 94.2%),。The inducement medium were as follow, respectively:for the leafstalk, B5 medium with 6-BA 0.5mg/L+IBA0.1 mg/L, and for stems, B5 medium with 6-BA 1.0 mg/L+IBA0.2 mg/L, and subculture medium both were B5 medium with 6-BA1.0 mg/L +2,4-D0.1 mg/L.Through single factor and orthogonal experiment, this study ascertains the best condition of grape cell suspension culture was that during cell suspension culture,2 mL of peptone (1M) at the 3rd day and 4 mL of sucrose (1M) at the 8th day were added into the medium, respectively; at subculture,40% inocula were maculated to fresh medium; in all suspension cultures, the temperature was at 25℃, the shaking spreed was 110 r/min under darkness. At that time, the content of total resveratrol was 203.7μg/20mL, and the dry cell weight was 62.5mg/mL.In this research, screening the explants of grape, getting the relaxed callus and optimizing cell suspension culture medium and condition, it will provide industrialization production of resveratrol to accumulate preliminary paramete
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