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Selection of Excellent Strain Corylus Heterophylla Fisch and Study on Tissue Culture and Rapid Propagation

Author: HeWei
Tutor: CaoHouNan
School: Yanbian University
Course: Pomology
Keywords: Corylus heterophylla Fisch Excellent strain Tissue culture Rapid Propagation
CLC: S664.4
Type: Master's thesis
Year: 2009
Downloads: 29
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Abstract

The Corylus heterophylla Fisch is a kind of fine nut fruit tree as well as an important tree in north ecological-economic forest construction. In our country, Corylus heterophylla Fisch trees are widely distributed, but it has not been reasonable development and utilization. And the yield per tree is low. The nut quality is also inferior. So it is necessary to select and improve new varieties. At the same time, there are only a few excellent strain of selection. But the traditional vegetative propagation can not supply production demand since low layering propagation ratio. It is important that rapid propagation by tissue culture. This research studied on the improved variety selection and breeding of Corylus heterophylla Fisch through the rich idioplasmatic resources of Yanbian State. And we study on tissue culture and rapid propagation techology by using excellent strain of selection as the materials. The mains results are as follows:Mature period in seed mainly concentrated in late August and early September. The average nut weight between 0.57g and 2.48 g .The most of it between 1.25g and 1.50 g. only a few above 2.0g .The average nut weight has relativity with the kernel rate per plant, that was the higher average nut weight, the lower kernel rate per plant. But there was plus tree preliminary Selection which had higher average nut weight and kernel rate. Yield per plant of three-year-stub between 100g and 200g. The most range was 100g～200g But there were also yield per plant more than 600g, and fruit traits were good. There was Corylus heterophylla Fisch which had stronger cold tolerance and better health in anti-environment then hybrid hazelnut. It was More suitable for cultivation in Yanbian and cold regions of North.The average nut weight average kernel weight and aernel rate of excellent strain 06-34-1 were 2.12g ,0.78g and 37.0%. Nut size was uniformity and surface was clean. It had strong cold tolerance and adverse resistance, good health in environment of using herbicides. The plant was stout too. The yield per plant was above average, and it was 372.0g.The average nut weight average kernel weight and aernel rate of excellent strain 06-37-1 were 1.64g , 0.64g and 38.5%. The Nut surface was clean, thin shell. It had strong cold tolerance and adverse resistance. Fertility, and the yield per plant was 686.5g.The best explant was buds which growed on the greenhouse and shooted forth in March. In the initiation culture, the suitable medium for Corylus heterophylla Fisch was DKW+TDZ 0.2 mg·L^-+IBA0.01mg·L^-1+200mg·L^-1AC+sucrose30g·L^-1 + agar6.5 g·L^-1, if the explant was buds which growed in field and shooted forth in mid-April, it can be combination water culture for 6 days before inoculation. In the subculture stage, suitable medium for the proliferation of Corylus heterophylla Fisch was DKW + TDZ 0.2 mg·L^-1+IBA 0.10 mg·L^-1 + sucrose 30g·L^-1+agar6.5g·L^-1, the multiplication coefficient was 2.4. In the rooting stage, suitable medium for the rooting of Corylus heterophylla Fisch was l/2MS+NAA0.20+sucrose10·L^-1+ agar6.5g·L^-1, rooting rate was 70.0%. The survival rate was up to 52% when the plants had been transplanted in a mixture of humus and vermiculite （3:1）.

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