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Rapeseed is the main oil crops and a major source of edible vegetable oil that accounted for more than 50% in China. In the same genetic background, the high oleic acid include the advantages of suitable cooking, nutritional value, easy to translate into diesel and so on. Although oleic acid which come up to about 60% in general, which is the main fatty acids in erucic acid varieties of Brassica napus, it can not meet the needs of living and producting, thereby increasing the oleic acid content in rapeseed is an important breeding objective.This study is based on high oleic acid Brassica napus cultivar H0810 and H0804 as a low-acid variety parents built 90 F2 generation of isolated groups, checking fatty acids of the F2 seeds by gas chromatography, then mapping oleic acid gene and QTL analysis by SSR markers in Brassica napus. The results obtained are as follows:1 High-and low-parent of the oleic acid content were 79.18%、57.14%, respectively. F1、F2 generation were 73.33%、73.59% in average oleic acid content, respectively. F2 was a single peak distribution groups （P = 0.99） through the distribution of the fatty acid data of F2 segregation population. The correlation analysison of fatty acid find that oleic acid with arachidic acid、palmitic acid, linoleic acid and linolenic acid show significant correlation, proving that there are multiple gene locis controling the content of oleic acid.2 Through 269 pairs of SSR primers screened group, We found 93 pairs of primers is different, but the FAD2 gene is located on the N5 linkage group in only five pairs of primers for genetic mapping. Four pairs of positioning in the genetic map were MR119, CNU398, CN53, BRAS095. QTL analysis of oleic acid find that a control QTL is between the CN53 and CNU398, and its LOD value is 4.83, the contribution rate is 59.37%.3 With the SSR markers A genome genetic map linkage group N5 （http://www.geboc.org/） comparison in Brassica napus, We find the common markers MR119 and BRAS095, indicating that they have a colinearity.
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