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Construction and Application of Primary DNA Fingerprint Database of Rice Varieties in Zhejiang Province

Author: ChengBenYi
Tutor: ZhuangJieYun
School: Chinese Academy of Agricultural Sciences
Course: Crop Science
Keywords: Rice variety Microsatellite marker Genetic diversity DNA fingerprint database Distinctness
CLC: S511
Type: Master's thesis
Year: 2009
Downloads: 28
Quote: 0
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Abstract


The study was conducted to analyze the genetic diversity of the major commercial rice varieties in Zhejiang province.44 conventional rice varieties and 54 hybrid rice combinations in Zhejiang province were assayed with a set of 12 microsatellite markers. We detected a total of 68 alleles and 127 fingerprints from all tested varieties, ranged from 2 to 10 with an average of 5.7 and 3 to 18 with an average of 10.6 per marker, respectively. The values of the frequency of polymorphism ranged from 0.567 to 0.905 with an average of 0.752. The results of genetic diversity analysis indicated that conventional rice varieties and hybrid rice combinations both had obviously genetic difference among subspecies, but they had less genetic difference within subspecies. Genetic diversity of indica type was higher than that of japonica type. The AGSC (average genetic similarity coefficient) of conventional indica, japonica and indica x japonica rice varieties were 0.672,0.711 and 0.103, respectively. The AGSC of hybrid indica, japonica, and indica x japonica rice combinations were 0.638,0.669 and 0.343, respectively. Cluster analysis showed that the conventional rice varieties and hybrid rice combinations were classified into 6 and 7 groups respectively, most of varieties or combinations with same type gathered into same one group.A total of 98 major rice varieties in Zhejiang province from 2002 to 2006 and 155 rice varieties in Zhejiang provincial regional trial from 2007 to 2008 were assayed with a set DNA fingerprint testing system on rice variety. A DNA fingerprint database containing genotypic data of 279 rice variety times at the 12 marker loci was constructed. The polymorphism of different markers and genetic similarity of varieties in the DNA fingerprint database were analyzed in detail. Based on the DNA fingerprint database, the distinctness of all rice varieties and consistency of the two-year regional trial varieties in different years were identified. It was found that 5 groups containing 11 rice varieties had no obviously distinctness within groups, the fingerprint data of 1 two-year regional trial variety between two years was not consistent. The result of planting trial in field showed that it was feasible to use DNA fingerprint technology and constructed DNA fingerprint database to identify the distinctness of rice varieties and consistency of two-year regional trial varieties in different years in Zhejiang province.

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CLC: > Agricultural Sciences > Crop > Cereal crops > Rice
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