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This research is for our country several common medicinal bryophytes greenhouses cultivation and biological characteristics of basic research, the current coverage for the cultivation of moss still small, especially in the interior areas reported less cultivated, this study in order to carry out the artificial cultivation of moss, lichen development and use of these medicinal provide background information. Select Hypnum Branch (Hypnaceae) big gray moss (Hypnum plumaeforme Wils.), Thuidiaceae (Thuidiaceae) Thuidium (Thuidium cymbifolium (Doz.et Molk.) Doz et Molk), Polytrichum Branch (Polytrichaceae) Blonde moss (Polytrichum commune Hedw.) 3 species of medicinal moss artificial cultivation experiment, respectively, considering the different culture medium, light intensity effects on the growth conditions, soil culture conditions, spray MS, Knop, White 3 seed medium for large gray moss, Oba moss Polytrichum growth, and for three kinds of moss gametophyte liquid culture were studied. And light intensity on the substrate growth of moss trials, set humus, sand, perlite three kinds of substrate and 25%, 50%, 75%, no shading four kinds of light intensity in the absence of three species of shade Moss can not grow normally, except for light intensity is 75% obscuration, the peat, perlite, sand these three kinds of substrates on the growth of influential big gray moss, the other three kinds of light intensity matrix on the big gray moss Oba moss Polytrichum growth no significant impact. Light intensity in 3 during the growth of moss has played a very important role in different light intensity, the three growth of mosses have shown significant differences, experimental results show that the 25% shading conditions most favorable to big gray moss, Oba moss Polytrichum growth. Spraying the liquid culture growth of moss of three trials, MS, Knop, White 3 seed culture liquid were set pH 5.5,6.5,7.5 process 3, the test results show that: Three culture solution at pH 5.5, respectively, 6.5, 7.5 treatments for large gray moss, Oba moss Polytrichum no role in promoting growth, but the spray handle different pH values ??in the culture medium of three moss chlorophyll content differences. In three species of bryophytes gametophyte liquid culture test, MS, Knop, White 3 seed medium pH value is set to 3.0-12.0, pH gradient of 0.5, the test results showed that: in liquid culture under three kinds of bryophytes on the pH of the culture medium values ??are different adaptation range. Large Hypnum liquid MS medium in the normal growth pH range of 3.5-8.0; Knop medium in the pH range of normal growth of 3.0-11.0; in White under normal growth medium in the pH range of 3.0-10.5 . From the growth length view, White culture medium at pH 5.5, 6.0 and 6.5, and under treatment Knop medium at a pH of 6.5 with the next big gray moss grow longer than the control group, and the control group there was a significant difference; Oba in MS medium moss grow normally in the pH range of 4.5-8.0; in Knop medium pH value under normal growth 3.5-11.0; in White normal growth medium pH of 3.0 to 11.0. From the growth point of view the length of the three different pH culture solution treatment on the growth Thuidium have no promoting effect; Polytrichum liquid MS medium in the normal growth pH range of 4.0-8.0; in Knop medium under normal growth the pH 3.0-10.5; in White under normal growth medium pH of 3.0-10.0. From the growth length view, White culture medium at a pH of 4.0, 4.5, and under treatment Knop medium at a pH of 4.5 with the next length of Polytrichum growth exceeded the control group, and the control group there was a significant difference. From the chlorophyll content point of view, big gray moss on MS medium pH 5.5 and treated under the highest chlorophyll content, Thuidium culture medium in three kinds of processing under different pH values ??were not exceeded chlorophyll content, chlorophyll content in Polytrichum Knop medium pH 6.0 with the next highest.
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