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The grain selenium （Se） densities among various genotypes, high zinc and iron contents for genetic utilization from Israeli wild emmer wheat, Triticum dicoccoides, were investigated in the present study. The obtained results showed that the grain Se densities of 110 genotypes of the tetraploid wild progenitor of wheat originating from 15 populations in Israel and 51 wheat cultivars from Guizhou and other provinces in China were detected by hydride generation atomic fluorescence spectrometry （HG-AFS）. The results showed that there were significant differences of grain Se density among populations by one way ANOVA （P≤0.001）. Grain Se concentration （GSeC） and grain Se content among the 110 wild emmer wheat genotypes varied from 0.043 to 0.409 mg kg-1 and 0.0008 to 0.0125 ug seed-1, with the average of 0.180 mg kg-1 and 0.0046 ug per seed, respectively. The highest genotypes of GSeC and grain Se content were TZ36 and TZ34, both derived from Bat-Shelomo population, while the lowest genotypes were TZ120 from Gamla and TZ8 from Mt. Hermon, respectively. The coefficient of variation of GSeC among populations also was obviously different, ranged from 9% （Bat-Shelomo） to 74% （Givat-Koach）. According to Spearman’s Rho Correlation analysis, the GSeC had significant negative correlationships with altitude, mean annual rainfall, mean number of dry days in original area, and positive correlationships with mean annual temperature, mean temperature in August, mean temperature in January and soil types, respectively. The whole level of cultivars GSeC is from 0.018 to 0.363 mg kg-1 lower than Triticum dicoccoides and the difference of GSeC among regions and varieties is not significant. The grain Se content had similar tendency of GSeC. These demonstrated that natural selection has created abundant phenotypes of grain Se density in this wild species, and would be used for identification of novel genotypes or genetic studies on wheat mineral nutrition.Meanwhile, four individuals carrying the high zinc and iron characteristics from the gene GPC-B1 , i.e., a strain of wheat GPC, R-GPC, PF638741, PI638740 （BC6F3） as the male parents crossed with Guinong 775 , Xingmai 2 and other four cultivars with no GPC-B1 gene as the female parents. Then, the F1 and the backcross generation were obtained. Using SSR markers linked closely to the GPC-B1 gene, the parents, F1 and BC1F1 individuals were analyzed in this study. The results showed that the four American GPC B1 individuals, nine F1 and eleven BC1F1 were found the band special to the GPC-B1 gene, and all cultivars from Guizhou were not occurred this novel band. The obtained results also showed that for the grain Zn concentration （GZnC）, PF638741 was the highest, 65.9mg Kg-1 among the detected materials, for the grain Fe concentration （GFeC）, PI638740 was the highest, 74.6mg Kg-1. GZnC and GFeC of most F1 individuals were 10%-40% higher than those of parents. And BC1F1 individuals also showed the same trend. These indicated that GPC-B1 transgenic wheat is beneficial to reuse the mineral Fe and Zn from the flag leaf to the grain accumulation, which led to increase the GZnC and GFeC.
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