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Rotation SOP of Rhizoma Cyathula and Rhizoma Coptis and Study on the Extraction and Isolation Technique of Polysaccharide from Rhizoma Cyathula
Author: ZhaoLei
Tutor: WangShuLin
School: Chengdu University of Traditional Chinese Medicine
Course: Pharmacognosy
Keywords: Cyathula Coptis Rotation Polysaccharide Extract
CLC: S567.239
Type: Master's thesis
Year: 2009
Downloads: 65
Quote: 0
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Abstract
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The thesis is divided into two parts, the first the \\water quality and soil ) were detected , the results show that the base environment to meet planting requirements ; through the histomorphological study to find out to identify the characteristics of the two aspects of plant morphology the Cyathula and hemp Achyranthes original and medicinal traits ; pair Cyathula seed quality grain weight and germination rate the seed weight 1.42g ~ 1.50g, the germination rate of 34% ; were carried out crop rotation , continuous cropping and the ordinary planting three models Cyathula emergence time , fertilizer trials , two main A comparative study of the disease ( white rust and root-knot nematode disease ) , preliminary draw Cyathula , in Emei longchi can with Coptis rotation . \, the optimum concentration of alcohol precipitation , extraction times of 1 to 85%. The crude polysaccharide Cyathula the after Sevage method three times in addition to protein , H2O2 combined dialysis method addition to pigment and small molecule impurities , and then after DEAE-52 cellulose and SephadexG-25 separation to obtain a polysaccharide component of COP I -1 by high performance gel permeation chromatography (HPGPC) Determination COP I -1 purity , molecular weight, the result is : COP Ⅰ -1 higher purity , and a weight average molecular weight of 4572.8 . Thin layer chromatography preliminary study COP I -1 monosaccharide composition of D-glucose and D-fructose .
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