Dissertation > Excellent graduate degree dissertation topics show

Expression, Characterization and Antigenicity Evaluation and 3D Structural Modeling of Peanut Allergen iso-Ara h 3

Author: ZhongHaiFeng
Tutor: MaSanMei
School: Jinan University
Course: Biochemistry and Molecular Biology
Keywords: Peanut allergens Cloning and expression Immune identification Antigenicity Three-dimensional structure
CLC: S565.2
Type: Master's thesis
Year: 2009
Downloads: 47
Quote: 0
Read: Download Dissertation

Abstract


Food allergy is considered a more serious nutritional problems , has aroused widespread concern . Peanut ( Arachis hypogaea.L ) is a nutritious protein resources . At the same time , in the world, FAO ( FAO , 1995 ) reported eight categories of food allergens , peanut allergens is important . Peanut allergy with a low dose sensitization, high mortality and life along with other characteristics, and therefore also been extensively studied . In this study, Touch down PCR technology , from peanut cDNA clone the peanut allergens iso - Ara h 3 gene , the results obtained two clones were named of PN32 - 8 and PN32 - 20 . Sequencing and alignment analysis , initially identified as the - clone basis because the target gene , and the target gene into the expression vector pET-44a was transformed into E. coli induced expression . Target gene expression by SDS-PAGE confirmed the 58 kDa protein , Western blot technology while taking advantage of the recombinant protein antigen identification . The results showed that recombinant iso - Ara h 3 protein serum recognition rate of 12.5% ??, and peanut major allergen the original Ara h 3 is a weak compared to the allergenic protein . Finally, bioinformatics antigenic analysis of the recombinant protein , and in the three-dimensional structure of the successful implementation of single amino acids virtual transformation .

Related Dissertations

  1. cDNA Cloning, Expression of vp5 and vp7 Genes and Subcecullar Localization of VP5 and VP7 Proteins in Grass Carp Reovirus,S941.41
  2. Cloning, Expression of vp6 and ns38 Genes and Immunogenicity of VP6 and NS38 in Grass Carp Reovirus,S941.41
  3. Isolation and Characterization of Carbendazim-Degrading Strains, Cloning and Expression of the mheI Gene,X172
  4. Pathogenic Mechanism of Invasion Associated Genes in Avian Pathogenic E. Coli Duck Isolate DE205B,S852.61
  5. Isolation, Identification and Degrading Characteristic of a Cyhalofop-Butyl-Degrading Bacteria, and Cloning, Expression of a Cyhalofop-Butyl-Hydrolyzing Gene chbH,X172
  6. Cloning and Expression of Organic Solvent Tolerant Lipase Gene from Staphylococcus Saprophyticus M36,Q78
  7. Screening of Bacterial Strains with High α-Galactosidase Activity and Its Gene Cloning, Expression, Purification and Characterization,TQ925
  8. Effects of Diclazuril on G3PDH in Second-generation Merozoites of Eimeria Tenella,S858.31
  9. Molecular Biological Characteristics of Multiple Porcine Fc Gamma RⅡB Sub-isoforms Generated by Alternative Splicing,S828
  10. Identification of New Protective Antigens of Streptococcus Equi SSP. Zooepidemicus,S855.11
  11. The Cloing、Expression and Initial Assessment of Immunoprection of Thioredoxin Glutathione Reductase of Schistosomiasis Japonicum,R392
  12. Study on Model-Based Visualization of Wheat Root Systems,S512.1
  13. The Proteomics Analysis of Response to Cadmium Stress in Radish (Raphanus Sativus L.),S631.1
  14. Molecular Cloning, Sequence nad Expression Analyses of Two Transcription Factors ZmC4HC3 and ZmNAC,S513
  15. Cloning and Expression Analysis of Cold-Related Genes of Ammopiptanthus Nanus,S793.9
  16. Invasive plant Eupatorium cold acclimation ICE1 gene cloning,S451
  17. Molecular Cloning and Prokaryotic Expression of Chalcone Synthase Gene in Fressia Hybrida,Q943.2
  18. Cloning and Prokaryotic Expression of avenin-like b from Wheat Variety Zheng 9023,S512.1
  19. The Isolation and Genetic Transformation of WRKY Gene in Wheat(Triticum Aestivum L.),S512.1
  20. Hemp bast fiber degumming Preparation and characterization of alkaline pectinase,TQ925.3
  21. Prokaryotic Expression and Renaturation of Streptavidin,Q78

CLC: > Agricultural Sciences > Crop > Economic crops > Oil crops > Peanut
© 2012 www.DissertationTopic.Net  Mobile