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Potato as a staple food crop has been widely planted in China. Potato virus disease affecting potato yield and quality is an important factor, especially potato virus Y (PVY), is harmful to the economy of China's potato production one of the most serious viral diseases, but their national identity and diversity of the virus rarely For potato virus research in Hunan is blank. This paper aims to infection of potato virus Y 6 Hunan isolates (HN1-6) to detect and study its molecular typing. The main results are as follows: 1, based on RT-PCR technique, for the first time in the country discovered and identified two recombinant PVY strains. HN1, HN4, HN5, HN6 similar to the European model of potato tuber necrotic ringspot strain line (Eu-PVY NTN ), with a common strain (PVY o ) and European type Tobacco vein necrosis strain (Eu-PVY N ) of three typical recombination sites (RJ1-3); while HN2 and HN3 only to find RJ 1 and RJ < sub> 2 two recombination sites. 2, this representative isolates of PVY-HN2 complete gene sequences were cloned and sequenced, with PVY O and Eu-PVY N sequence comparison, results show HN2 also has a similar PVY NTN -Hun same three recombination sites, these two strains isolates in RJ 2 (nt 5821) Restructuring interval consistent, but in RJ 1 (PVY NTN -Hun vs. HN2, nt 2421 vs.2521) and RJ 3 (PVY NTN -Hun vs. HN2, nt 9221vs.8581) the location of recombination sites interval changed. 3, for the new RJ 3 design a new RT-PCR primer pair, the establishment of a new identification RJs method of RT-PCR analysis. Use of this new and improved detection methods, for all isolates tested Hunan. Found HN4 and HN5 are essentially two kinds of complex recombinant PVY strains infect. This result further confirms the effectiveness of detection. 4, identified by ELISA, Hunan isolates of potato virus Y strains. With DAS-ELISA test isolate whether PVY N strains showed that recombination sites with three similar Eu-PVY NTN samples HN1, HN4, HN5, HN6 positive. With TAS-ELISA detects whether the isolates PVY o strains showed that with RJ 1 and RJ 2 two recombination sites of the sample HN2, HN3, HN4, HN5 positive. The results are consistent with the molecular characteristics.
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