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Mechanisms of Exogenous NO on Resistance of Arabidopsis to Grey Mold Disease

Author: ZhaoXiaoDan
Tutor: YangHongYu
School: Yunnan Normal University
Course: Biochemistry and Molecular Biology
Keywords: Arabidopsis Botrytis cinerea disease resistance nitric oxide hydrogen peroxid
CLC: S432.4
Type: Master's thesis
Year: 2008
Downloads: 14
Quote: 1
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Abstract


Grey Mold Disease caused by Botrytis cinerea is one of the main plant diseases. In recent years, it becomes more and more serious and causes enormous economic loss. Here we discuss the effect of exogenous nitric oxide (NO) on resistance of Arabidopsis to the grey mold disease and its possible mechanisms. This study results will be helpful at revealing the biochemical mechanisms of NO in crops protection against grey mold disease in agriculture. The main results are summarized as follow:1. Plant leaves were inoculated with fungal conidia after treated with SNP spray on leave surfaces. We found that SNP spray could reduce the incidence of grey mold disease. We used various concentrations of SNP to treat the Botrytis cinerea in our experiment. The results showed that the lower concentrations of SNP have no inhibitory effect on the growth of Botrytis cinerea. This suggests that the decreased disease incidence of Arabidopsis was due to exogenous NO-induced resistance. The optimal concentration of SNP inducing plant resistance to the pathogen is 0.5 mmol/L. And the best interval of SNP treatment is 3 days. But we found that the higher concentrations of SNP obviously have directly inhibitory effect on the growth of Botrytis cinerea. So do to the plant.2. Exogenous NO reduced the accumulation of Reactive oxygen species(ROS). It also inhibited hydrogen peroxide (H2O2) bursting and decreased disease incidence of the tested plant.3. Plant leaves were treated with SNP spray only or inoculated with fungal conidia after sprayed with SNP, the activities of catalase(CAT), proxidase (POD) and phenylalanine ammonia lyase (PAL) all were increased obviously except sodium dodecyl sulfate(SOD). These results indicated that activation of CAT and POD which resulted in the reduce of the accumulation of H2O2 probably is the causes of decreasing disease incidence in the plants. Then POD and PAL are related with the synthesis of lignin. And the increase of their activities resulted in improving the content of lignin which can prevent plants from pathogens invading. But no obvious change of SOD was checked in our study, so the major reasons which cause plant disease probably are superoxide anion radical(O2).

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CLC: > Agricultural Sciences > Plant Protection > Pest and Disease Control > Plant diseases and their prevention > Transgression ( pass ) an infectious agent harmful
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