Dissertation > Excellent graduate degree dissertation topics show

Construction of Mimic Artificial Blood Vessels with Catalyzing No Generation

Author: LiYanDong
Tutor: WuWenJie
School: Tianjin University of Science and Technology
Course: Applied Chemistry
Keywords: Self-assembly Bionic Nitric oxide Catalytic generation Artificial vessels
CLC: R318.11
Type: Master's thesis
Year: 2011
Downloads: 8
Quote: 0
Read: Download Dissertation

Abstract


In this paper, the method to fabricate artificial blood vessel was researched to simulate natural vascular structure. Because of the capacity that organic selenium can catalyze endogenous NO donor RSNOs decomposing to release of NO, organic selenium compound was loaded onto the surface of artificial vascular scaffold. The bioactivity of the modified material was evaluated by catalytic generation of nitric oxide, platelet adhesion and kinds of cellular behavior experiments. The feasibility of building implanted vascular scaffold with bionic catalytic controlled generation of NO to obtain anticoagulant property and accelerate endothelialization was further examined.Organic selenium catalyst SePEI were synthesized and characterized, and then used as polycation with PGA as polyanion coated onto the surface of polycaprolactone (PCL) nanofiber scaffolds obtained by electrospinning layer-by-layer. The LBL structures were characterized. Catalytic generation of NO from the NO donors-RSNOs was tested in the existence of reducing agent RSH. Biological properties were also evaluated, such as cell proliferation experiments and anti-coagulation experiments.It can be seen from the infrared spectrum that the amide bond is formed, and there are still a lot of amine groups to ensure water-soluble of the production. The amount of Se element loaded on SePEI is also detected by atomic absorption. The self-assembled layers are characterized qualitative and quantitative by UV-vis and atomic absorption spectrometry. With the increasing of numbers of the layers, Se element content is also added. The laciniation of the contact angle represent the alternating adsorption of polycations and polyanions. It is shown in the catalytic releasing NO test that the level of the release amount of containing Se group is significantly higher than that of control group, indicating that Se has the catalytic function. The NO release was relatively stable with no significant burst appeared, and still could be detected at the80hours. The survival rate of fibroblasts and smooth muscle cells on the surface of the materials are tested. Those confirm that the materials show little cytotoxicity, and have the effect of inhibitting proliferation of smooth muscle. The anticoagulant property of catalyzing NO-releasing grafts (SePEI/PGA)10was characterized by using the AV-shunt experiment. Through the stereo microscope, HE staining and scanning electron microscopy, experiment materials maintain clean and smooth surface and don’t produce acute thrombosis, and the amount of the platelet on the surface of Se containing groups are significantly lower than the control groups. This new style of stent material is capable of catalyzing the NO donor releasing NO. The NO release is relatively stable with no significant burst appeared, and can last for a long time.

Related Dissertations

  1. Studies on the Effects of Exogenous Nitric Oxide on Rice under Cadmium Stress,S511
  2. The Relationship between Exhale Nitric Oxide and Serum Eosinophil Cationic Protein, IL-13, IL-8 in Acute Asthma Exacerbation,R562.25
  3. The Relationship of Serum Adiponectin、Nitric Oxide Synthase and Carotid Atherosclerosis in Patients with Acute Cerebral Infarction,R743.33
  4. Soft tissue crush injury in rats induced reactivity of isolated thoracic aorta changes,R686
  5. O Single- amino- phenyl-substituted porphyrin derivatives and research,O626
  6. Study on the Effect of Gold Nanoparticles in the Immunological Response Induced by Lipopolysaccharide,R318.08
  7. Substituted phthalocyanine synthesis and molecular self-assembly behavior,O621.3
  8. Fabrication and Electrochemistry of Two-dimensional Ordered SiO2 Cavities on Electrode Surfaces,O613.72
  9. The Physiological and Biochemical Changes Induced by Polyamines and Nitric Oxide During Lettuce (Lactuca Sativa L.) Seed Germination,S636.2
  10. Reference Values and Influence Factors of Fractional Exhaled Nitric Oxide for Healthy Children in Soochow,R446.1
  11. The Study of the Self-assembly Behaviors of Some Chiral Molecules with Fluorocarbon Chains,O621.3
  12. The Mechanisms of Propofol-induced Relaxation on Isolated Thoracic Aorta Rings in Rats,R614
  13. In Vitro Study of Theeffects of Fullerol on the Hippocampal Synaptic Plastisity,R741
  14. Fabrication and Property of Films Based on Polyoxometalates and Dyes,O611.3
  15. Research on Bionics Design of Car Contour Design,U462
  16. Study on the Physical Strength of the Change and Improvement in OCC Recycled Processing,TS71
  17. Effects of Acute Phase Serum of the Children with Kawasaki Disease on the Human Umbilical Vein Endothelial Cells Function,R725.4
  18. The Preparation and Performance of Funcational Materials Based on Polymer,TB34
  19. Oxidation Removal of Sulfur Dioxide and Nitric Oxide by Ozone in the Flue Gas,X701.3
  20. 4-Amino-1,8-naphthalimide Based Fluorescent Probes for ROS: Design, Synthesis and Property,O621.2
  21. Design and Control of Intelligent Bionic Leg Base on Hybrid Actuation,TP242

CLC: > Medicine, health > Basic Medical > Medical science in general > Biomedical Engineering > Artificial organs and organ > Department of Cardiovascular
© 2012 www.DissertationTopic.Net  Mobile