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Study on Biosensors Based on LSPR of Noble Nanoparticles

Author: YangJiaoFeng
Tutor: LiuXianHua; YangJian
School: Tianjin University
Course: Environmental Engineering
Keywords: LSPR noble metal nanoparticles self-assembly Electrochemicaldeposition detection plaform
CLC: TP212.3
Type: Master's thesis
Year: 2012
Downloads: 5
Quote: 0
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Abstract


Localized surface plasmon resonance （LSPR） properties of Au nanoparticles havebeen studied sufficiently in recent years in the field of biosensors. Compared withtraditional SPR biosensors, the LSPR biosensors have certain advantages such as lowcost, miniaturized device, portable, etc., which make it promising candidates in thefield of environmental monitoring, medical treatment, food analysis, etc. The mainresearch focus is to increase LSPR sensor’s sensitivity since it shows low sensitivity.Au/Ag nanoparticles possess unique optical, electrical, and magnetic properties,which are the most common used nanomaterials in LSPR sensors. The purpose of thisstudy was to prepare LSPR biosensors, self-assembled Au/Ag nanoparticles LSPRchips through wet-chemical and electrochemical method, and finally designed a kindof in situ preparation and online detection LSPR biosensor device.First, the triangle Ag nanoparticles were prepared using wet-chemical method atroom temperature, which side length was between50-80nm, and the depth wasbetween5-15nm. The result showed that hydrogen peroxide plays a key role duringthe process for Ag nanoparticles changing to triangle shape. APTES was used aslinkage for providing amino group, which can self-assemble triangle Ag nanoparticlesonto the surface of glass substrate. Sucrose solution was served to characterize Agnanoparticles’ refractive index sensitivity to surrounding medium. The measuredrefractive index sensitivity was as high as247nm/RIU.Second, the LSPR biosensor devices which can in situ preparation and onlinedetection was designed and prepared, which minimized the influence of movement todetection result. The device can also realize device miniaturization, online detection,and easy operation.Third, The Au nanoparticle LSPR chips were prepared using constant potentialelectrodeposition method in this kind LSPR biosensor device. The refractive indexsensitivity increased first and then decreased with deposition voltage, meanwhile theabsorbance shows linear relation with surrounding medium’s refractive index’schange. According to our results, the possible mechanism of electrochemicalself-assembled Au nanoparticles was divided into three stages. At the second stage, the reversible reaction occurred between zero valent gold and gold ions, which hadre-adjusted the distribution of the chip surface of gold nanoparticles.Finally, the constant potential electrodeposition method was used to prepare Agnanoparticle LSPR chips in this device. The experimental results showed that whenthe potential was-1.4V, the thickness of ITO films was between23±5nm, the LSPRchips’ refractive index sensitivity reached246nm/RIU, which was as high as LSPRchips’ refractive index sensitivity prepared by chemical method in this study, andeven higher than Ag nanospheres and NSL method prepared Ag nano arrays that havebeen reported. The as-prepared Ag nanochips were used to detect biotin-avidin system,which show good response to avidin. It presented a liner response in the avidinconcentration range between1.5*10-10and1.5*10-6M （R2=0.9933）, whichestablished the LSPR biosensors detection platform for the interaction betweenbiomolecules.

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CLC: > Industrial Technology > Automation technology,computer technology > Automation technology and equipment > Automation components,parts > Transmitter ( converter),the sensor > Biological sensors,medical sensors
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