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Indirect Effects of Cypermethrine Administered to Spodoptera Exigua Larvae at Sub-Lethal Levels on Meteorus Pulchricornis (Braconidae, Hymenoptera)

Author: ShiZhenHua
Tutor: LiBaoPing
School: Nanjing Agricultural College
Course: Agricultural Entomology and Pest Control
Keywords: Virulence Host Selection Sublethal effects Pulchricornis gifuensis Beet armyworm Biological control
CLC: S476.3
Type: Master's thesis
Year: 2009
Downloads: 17
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Beet armyworm (Spodoptera exigua Hiibner) is one of the important pests of crops in China. Due to the resistance or resistance, many conventional chemical pesticides can not reach the control effect. Therefore, how to coordinate with chemical control become the comprehensive management of key issues. Virulence role on the basis of measured cypermethrin (Cypermethrine) overhang the Braconidae (Meteorus pulchricornis Wesmael) of the beet armyworm larvae and stains, research pulchricornis Braconidae drug host selection behavior observed by the parasitic drug host offspring bee fit features. The results for application pulchricornis the Braconidae and cypermethrin collaborative role in controlling pests, has a certain reference value. The main results are as follows: 1. Cypermethrin pulchricornis the Braconidae and beet armyworm virulence determination of concentration of cypermethrin liquid processing to 100 mg / L, 300 mg / L, 500 mg / L, 800 mg / L and 1000 mg / L. Toxicity Determination of membrane method, the Braconidae adults pulchricornis contact with liquid 48h mortality were 48.33%, 60.00%, 60.00%, 83.33% and 91.67% respectively. Micro-drip method 4th instar larvae of the beet armyworm virulence test showed that the beet armyworm has a strong lethal effect by the drug, the 4th instar larvae of the beet armyworm 48h mortality rates were 61.67%, 60.00 %, 86.67%, 98.33% and 100.00%. The measurement results provide a basis for processing Filter sublethal dose. 2 pulchricornis Braconidae accept the cypermethrin processing beet armyworm larvae selective two experimental studies were carried out pulchricornis Braconidae parasitic selective drug host larvae, parasitic selection experiments. 300 mg / L (concentration of the beet armyworm LC5) of cypermethrin liquid to trace the drip method contact beet armyworm larvae survival divided into not to pupation host (host larvae before pupation or parasitic bee pupa before death the host) and pupation host (host larvae successfully pupate or parasitic wasps complete the immature stage of development after successful pupation host), parasitoids different host host-selection. The parasitoids pupate host the parasitism rate (55.2%) was significantly higher than and not the pupation host (14.50%); probability with weight gain and improve host survival. The the coordinated control effect of parasitic and cypermethrin (80.40%) was significantly higher than the parasitic (54.00%) and cypermethrin (53.00%). The second study was designed according to the parasitic wasps parasitic identify host (identification period), the analysis of the risk of drug host larvae parasitized. Cox proportional hazards model relatively healthy and drug host larvae are parasitic of risk, the results show that, stains hanging Braconidae first parasitic, healthy host and the drug host the risk of parasitic no significant differences; hanging Braconidae but stains parasitic host parasitized by the drug significantly higher risk than healthy host. When the the parasitic wasps acupuncture object are healthy host, the identification of significant differences, but also significantly related (52.8%). This shows that pulchricornis Braconidae different time according to the the first acupuncture object and host evaluation result with a certain tendency in the 2nd acupuncture. When the the parasitic wasp first acupuncture object is healthy host, more inclined to and acupuncture health again host. In a short time, parasitoids easier acupuncture by the drug host; was a long period of time, it is easier to acupuncture host health. 3 descendants of parasitic drug by the beet armyworm larvae bee development of suitable degree performance pulchricornis the Braconidae parasitic drug beet armyworm larvae output offspring bee, bees was no significant difference in parasitism rate parasitic healthy host offspring , but the offspring bees development (cocoon rate, 88.54%) and body size (with hind tibia length and 1.66 mm) by a significant adverse impact. Based on the findings speculated that the administration of sublethal concentrations of pesticides such as cypermethrin against beet armyworm larvae, parasitic wasp parasitism has little effect.

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CLC: > Agricultural Sciences > Plant Protection > A variety of control methods > Biological control > The use of parasitic insects
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