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Research of Ear Allotransplantation in Rabbits

Author: LiuPing
Tutor: LiuBin;LiuYi
School: Lanzhou University
Course: Pathology and Pathophysiology
Keywords: Allograft Auricle reconstruction Immunosuppression immunologic reaction
CLC: R764
Type: Master's thesis
Year: 2008
Downloads: 5
Quote: 0
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Abstract


Objective:1.To establish an animal model of ear allo transplantation in rabbit and to investigate the pathologic changes of rejection after transplantation.2.To find an effective therapic strategy of immune suppression for improvement of the survival rate of allotransplanted ear and rejection.Methods:Livid blue rabbits and Japen big ear white rabbits were used as experiment animal.A free right ear based on common auricularis magna artery with auricularis magna vein and auricularis edge vein was harvested from donor(livid blue rabbit), then allotransplanted to recipient(Japen big ear white rabbits) with microvarscular anastomosis technique.40 couples of rabbits were divided into five groups in random according to different scheme of immunosuppressive treatment.Group O(n=10) and group C(n=5) were auto-transplantion and allo-transplantation without application of immunosuppressive agent respectively.Group T1,T2 and T3(each with ten couples of rabbits,n=10) were treatment groups of FK506,FK506 combined with prednisone and FK506 combined with prednisone plus MMF respectively.Then,the survival rate of transplanted ears,the incidence rate of rejection and its histopathologic grades,the levels of IL-2,IL-4 and IL-8 in plasma and CD4+,CD8+ in lymph cells of donor rabbits were examined.Results:1.The blood supply of ear was rich after allotransplantation.2.At day 30,60 and 100 after allotransplantation,the incidence ratio of rejection and the grades of histopathological of skin biopsy specimens and the expression levels of IL-2,IL-4 and IL-8 were significantly lower in Group T3 than the other groups.The treatment of group T3 showed without serious side effects.Conclusion:1.An ideal animal model of ear allo-transplantation in rabbits is estabilshed.2.The combined of FK506,MMF and prednisone scheme is a effective therapeutic strategy to prevent rejection after ear allo-transplantation.

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CLC: > Medicine, health > Otorhinolaryngology > Otology,ear disease
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