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Objective: To investigate the expression of aquaporin (Aquaporin, AQP) and knee osteoarthritis (knee joint osteroarthritis, KOA) the formation and development of a correlation, and explore Danshen injection with sodium hyaluronate (Sodium Hyaluronate, SH) Intra-articular injection of rat early KOA Development of its joint AQP expression and to intervene through regulation of the expression of AQP KOA development feasibility for the treatment of KOA's looking for new targets. : 60-month-old SD rats were randomly divided into three groups: normal group (A), model group (B), the experimental group (C) A group without any treatment, B, C two groups of 40 Right knee by intra-articular injection of 4% sterile papain and 0.03mol / L L-cysteine ??solution, resulting in the KOA model B group after modeling without any treatment, allowed free movement in the cage ; C group since the day of the first model of intra-articular injection of sodium hyaluronate 0.06m1 and Danshen injection 0.06ml, once a week, a total of three injections. Choose for the first time after modeling 3d, 7d, 14d, 21d four-phase point, rats were observed behavior to learn whether there are changes, the rats were killed by decapitation, cut rat right knee articular surface of the medial femoral condyle cartilage specimens, knee cartilage general observation, and then observed the indicators require fixed, the slice after HE staining, Mankin's pathological scoring method analysis of articular cartilage pathology integral and immunohistochemical staining by image analysis system analysis AQP1 and AQP3 expression in the articular cartilage. Results: 1 gross morphological comparison shows the experimental group and the model group was no significant difference; 2, histological scores results show that the experimental group was better than the model group, the severity of the experimental group and Mankin's scores were lower than that in the model group (P lt; 0.05 ); 3, the normal group of AQP1 of AQP3 in expression of AQP3 in the expression level stronger than that of AQP1; AQP1 expression level of the model group and the normal group, the experimental group, no significant difference (P gt; 0.05), but the AQP3 expression levels were significantly higher than normal group and the experimental group (P lt; 0.05). Conclusion: normal or pathological case of knee cartilage in both AQP1 and AQP3 expression in rats, but the AQP3 expression of the model group significantly increased, which prompted us of AQP3 The high expression perhaps with the formation and development of KOA there is some closely linked. Salvia Injection Combined with better treatment of intra-articular injection of SH rats early KOA maintenance of normal cartilage construct, to reduce the degree of cartilage degeneration, and delay the development of osteoarthritis, their treatment KOA molecular mechanisms may also associated with down AQP3 expression levels related.
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