Dissertation > Excellent graduate degree dissertation topics show

Effect of CP-690550 on the Acute Rejection of Allogeneic Heart Transplantion in Mice

Author: MingFei
Tutor: XiangDaoKang
School: Zunyi Medical College,
Course: Cardiothoracic Surgery
Keywords: Heart transplant Rejection CP-690550 CsA STAT3 IL-12 Mice
CLC: R654.2
Type: Master's thesis
Year: 2009
Downloads: 12
Quote: 0
Read: Download Dissertation

Abstract


Objective: To study the mice after heart transplantation using specific Janus kinase 3 (Janus Kinase 3 JAK3) inhibitors - CP-690550 the transplanted heart signal transduction and transcription activation factor 3 (signal transducerand activator of transcription 3 STAT3) and cell the content factor IL-12 ( interleukin-12 ) ; the observed cardiac allograft survival time and changes in cardiac pathology , and to explore the mechanism of action of the mouse heart transplantation after acute rejection . Methods: cervical heterotopic heart transplantation model of allogeneic mice , were randomly divided into intervention group , the CsA ( ring cyclosporin A ) and control groups , and each group n = 6 . The intervention group and CsA mice the day of surgery and postoperative were treated with CP-690550 and CsA oral ( both 10mg.kg -1 d -1 < / sup >) to the transplanted heart arrest , the control group did not give oral medication record three groups of cardiac allograft survival time . Take the transplanted heart specimens were detected by immunoblotting (western blotting) specimen surface STAT3 ; enzyme-linked immunosorbent assay (ELISA) was detected specimens surface IL -12 ; Paraffin sections, HE staining was observed myocardial lymphocytic infiltration situation. Results: 1 . Intervention group cardiac allograft survival time (6.17 ± 0.75d) was significantly longer than the CsA group (4.67 ± 0.52d) and the control group ( 3.00 ± 0.63 d ) ( F = 36.62 , P <0.05 ) . 2 microscope transplanted heart (HE staining 200 times ) , myocardial lymphocytic infiltration of the CsA group and the control group than the intervention group seriously . 3 heart specimens in the intervention group phosphorylation of STAT3 content of the reference value (0.23 ± 0.05) was significantly less than the CsA group (0.29 ± 0.06) and control group ( 0.47 ± 0.07 ) ( F = 75.39 P <0.05 ) . The intervention group heart specimens IL -12 content (142.24 ± 34.10pg/ml) significantly less than the CsA group (202.34 ± 64.10pg/ml) and control group ( 282.03 ± 51.89pg/ml ) ( F = 5.30 P <0.05 ) . Conclusion : CP-690550 by reducing phosphorylation in cardiac allograft active STAT3 content , thereby inhibiting JAK3-STAT signaling pathway , inhibition of dendritic cell maturation and release of IL-12 -induced T cell anergy , causing immune tolerance anti- acute rejection , thus prolonging cardiac allograft survival time , and better than that of CsA .

Related Dissertations

  1. Attitude Control of a Three-Axis Stabilized Satellite,V448.22
  2. The Research on Paper Currency Classification Method Based on Harr-Like Feature and Minimal Ball Including Samples,TP391.41
  3. Study of Sepia Ink-Astragalus Preparation for Relieving Damage Caused by Chemotherapy,R285.5
  4. Clinical Significance of STAT3 and MMP-2 in Hepatocellular Carcinoma.,R735.7
  5. Effects of Angiogenic Factor on Follicular Angiogenesis and Development in Immature Mouse,S865.13
  6. Study on Gene Expression of β-defensin1 in Mammary Gland of Mice,S852.4
  7. Roles of MMP-7 and Lysozyme in the Pathogenesis of Ulcerative Colitis Induced by DSS in Balb/c Mice,S858.91
  8. Some Pharmacology and Toxicity of Three Prescriptions from Chinese Veterinary Pharmacopoeia,S859.5
  9. Study of Melittin on Tumor Inhibition and Part of Mechanism of Human Hepatocellular Carcinoma HepG-2 Xenograft in Nude Mice,R735.7
  10. Apolipoprotein E Gene Knock-out and High-fat Diet on IP3、 IP3R-1 ＆ Aβ Expression in Neurons of Mice’s Hippocampal CA1 ＆ Ca3,R329
  11. Effects of TACI-Ig on MRL/lpr Mice and Activation of JAK1-STAT1 Signaling Pathway in Lupus Nephritis,R363
  12. Effects and Some Mechanisms of TACI-Ig on Immune Function in MRL/lpr Mice,R593.241
  13. Maternal Exposure to Fenvalerate in Embryo Accelerated Age-related Changes of Behaviors in CD-1 Mice,R714.2
  14. The Experiment Study Was Used Three Methods to Repair the Ligation of the Femoral Nerve,R651.3
  15. Effect of β-elemene Combined with Radiotherapy on the Angiogenesis of Lung Adenocarcinoma Xenograft Tumor in Nude Mice,R734.2
  16. Antitumor Effects of Elemene on the Proliferation Inhibition and Apoptosis Induction in Ascites Hepatoma Cell Line H22,R735.7
  17. The Experimental Research of RNAi Technology Inhibiting Respiratory Syncytial Virus,R725.6
  18. A Stuty on the Diversification of Interleukin-17 of Asthma Mouse and the Affection of the Treament of Glucocorticoids,R562.25
  19. Effects of Quercetin on Human Bladder Cancer Cells (EJ) and STAT3 Signaling Pathway,R737.14
  20. Gambogic Acid Blocks Proliferation and Induces Apoptosis of Lung Ddenocarcinom A549 Cells Through Downergulation of STAT3,R734.2
  21. Influences of Cisplatin on Expressions of P-glycoprotein in the Resistance Cells of in Lung Cancer Xenografts in the Mice,R734.2

CLC: > Medicine, health > Surgery > Of surgery > Cardiovascular and lymphatic system surgery > Heart
© 2012 www.DissertationTopic.Net  Mobile