Dissertation > Excellent graduate degree dissertation topics show

The Study of the Anti-stress-injury Effect of Moxibustion Pretreatment on the Rats’ Gastric Mucosa Through Inducing the High Expression of Heat Shock Protein 70

Author: PengNa
Tutor: ChangXiaoRong
School: Hunan University of Traditional Chinese Medicine
Course: Acupuncture and Massage
Keywords: Moxibustion Zusanli Liangmen Stress ulcer Gastric mucosal protection Heat shock protein 70 Gastric mucosal blood flow Malondialdehyde Endothelin Prostaglandin E2
CLC: R245-33.9
Type: Master's thesis
Year: 2006
Downloads: 6
Quote: 0
Read: Download Dissertation

Abstract


Objective:to explore the close relationship between the moxibustion’s effect of protecting gastric mucosa and the high expression of Heat Shock Protein 70 (HSP70) and the acupoints’relative specificity through observing the influence of the pretreatment of moxibustion on Zusanli (ST36) and Liangmen (ST21) on the stress-induced ulcer (SU) rats’gastric mucosa injury and comparing it with moxibustion on the non-acupoint.To clarify the internal mechanism of protecting the gastric mucosa of the acupuncture and moxibustion and its close relationship with Foot-Yangming meridian from the level of molecule.At the same time,to provide experimental evidence for the meridian-viscerus-correlati on theory and the clinical acupoint prescription.Methods:Sixty SD rats, both gender (by half), were evenly randomized into four groups:blank group, model group, Zusanli and Liangmen-moxibustion group and non-acupoints group.Preparing SU model using water restraint stress method, evaluating ulcer index (UI) by Guth method,determining the expression of HSP70 and HSP70mRNA of gastric mucosa with immunohistochemical and reverse transcriptase polymerase chain reaction(RT-PCR) technique,detecting the content of endothelin (ET) and prostaglandin E2 (PGE2) of gastric mucosa by radioimmunoassay and malondialdehydein (MDA) by thiobarbiturate technique.Results:1.Compared with blank group, rats’gastric mucosa UI in model group increased, the HSP70 expression and the content of MDA and ET also increased, the GMBF and the content of PGE2 decreased, with remarkable difference (P<0.01 or P<0.05).2. Compared with model group, rats’gastric mucosa UI in Zusanli-Liangmen group decreased, the HSP70 expression became more evident, the content of MDA and ET decreased, the GMBF and the content of PGE2 increased, with remarkable difference (P<0.01 or P<0.05).3. Except the content of ET, other detecting items of Zusanli-Liangmen group were remarkable different with those of non-acupoints group (P<0.01 or P<0.05).Conclusion:Moxibustion pretreatment on Zusanli and Liangmen could protect gastric mucosa. Its mechanism includes inducing gastric mucosa HSP70 and HSP70mRNA high expression, increasing GMBF, promoting PGE2’release and restraining MDA and ET producing, with the relative specificity of acupoints.

Related Dissertations

  1. Effects of Aspirin and Curcumin on COX-2, β-attention and PGE2 Expressions in Colon Cancer Cell Lines of HT-29 and SW 480 Cells,R735.35
  2. Studies on E-cadherin Expression Inducement of Prostaglandin E2 in HepG2 Cells and Its Molecular Mechanism,R735.7
  3. Study on the Pain Mechanism and Intervention Therapy of Chronic Nonbacterial Prostatitis,R697.3
  4. Expression and Clinical Significance of COX-2 and PGE2 in Lumbar Disc Herniation,R681.53
  5. Expression of β-catenin, COX-2 and PGE2 in the Carcinogenesis of Colorectal Mucosa in Rats,R735.34
  6. Sufentanil analgesia on rabbit blood dynorphin and prostaglandin E_2 research,R614
  7. COX-2 through Akt, NF-κB regulation of gastric cancer cell molecular mechanism of E-cadherin,R735.2
  8. Effect of Weilngchongji on the Level of Prostaglandin E2 and Hexosamine in Rats of Chronic Atrophic Gastritis,R285
  9. Investigation the Prophylactic Drug Therapy for the Pain during Root Canal Therapy and the Correlation between the Pain and the Level of PGE2 in Periapical Exudates,R781.05
  10. Study on the Effect of Celecoxib on the Growth and Angiogenesis of Orthotopic Transplantation Tumor of Experimental Human Colorectal Cancer,R735.35
  11. Celecoxib on human osteosarcoma cell line HOS-8603 Effect of,R738.1
  12. The Effects of Rebamipide on Tumor Necrosis Factor and Prostaglandin E2、Nitric Oxide in Rats with Acute Alcoholic Gastric Injury,R573
  13. Influence of Nimesulide on the Release of PGE2 and the Expression of Survivin in Human Tongue Squamous Cell Carcinoma Tca8113 Cell Lines,R739.8
  14. Investigating the Inflammatory Pathogenesis of Treating Asthmatic Guinea-pigs by Acupoint Application,R245.9
  15. Study on the Molecular Mechanism of Rat Bladder Carcinogenesis Induced by Terephthalic Acid,R737.14
  16. Analgesic Effects of Total Flavone of Rhododendron and Its Mechanism,R285
  17. The Expression of Four Kinds of Prostaglandin E2 Receptors and Their Intracellular Location in Human Liver Cancer Cells,R735.7
  18. Effect of Lornoxicam on Fos-protein, COX-2 of Spinal Cord and Plasma PGE2 in Incision-induced Rats,R965
  19. Influence of Cox-2 Inhibitor on Expression of Ang1 and Ang2 Gene in Human Tongue Squamous Cell Line Tca8113,R739.8
  20. The Clinical Significance of Prostaglandin E2 in Tissue and Peripheral Blood of Patients with Gastric Cancer,R735.2

CLC: > Medicine, health > Chinese Medicine > Chinese medicine clinic > Acupuncture, acupuncture therapy > Research Methods > Acupuncture with equipment and instruments
© 2012 www.DissertationTopic.Net  Mobile