Dissertation > Excellent graduate degree dissertation topics show

-13T/C Polymorphism of Saa 5’-Flanking Region in Ischemic Cerebrovascular Diseases

Author: ZhanJingJing
Tutor: ZhangChen
School: Qingdao University
Course: Neurology
Keywords: Serum Amyloid A Protein (SAA) Ischemic Cerebrovaschlar Diseases Polymorphisms
CLC: R743.3
Type: Master's thesis
Year: 2011
Downloads: 16
Quote: 0
Read: Download Dissertation

Abstract


Background and objectiveIschemic cerebrovascular diseases induced by atherosclerosis are serious hazard diseases affecting human health. As one of the pathogenesis of atherosclerosis, inflammation and its genetic predisposition play an important role in the development of ischemic cerebrovascular diseases. Serum Amyloid A Protein (SAA) is an acute inflammatory markers. This paper is to detect the association between -13T/C single-nucleotide polymorphisms (SNPs) at the 5’-flanking region of SAA and ischemic cerebrovascular diseases and to investigate the mechanisms by which SAA polymorphisms are involved by examining serum concentrations of SAA.MethodsUsed enzyme-linked immunosorbent assay (ELISA) to detect the serum levels of SAA and Matrix Metalloproteinases-9 (MMP-9) of 193 cases with ischemic cerebrovascular diseases (including cerebral infarction and transient ischemic attack) and 120 healthy. Used radioimmunoassay detected the serum levels of C-reactive protein (CRP). Analyzed single-nucleotide polymorphism -13T/C at the 5’-flanking region (rs:11024595) of DNA samples came from 291 cases with ischemic cerebrovascular diseases and 226 healthy by polymerase chain reaction (PCR) and gel electrophoresis imaging techniques.ResultsSerum levels of SAA are higher in ischemic cerebrovascular diseases subjects than in healthy subjects (t= 8.571,9.905,8.135; P<0.01). There is a positive correlation between SAA and CRP and MMP-9 in ischemic cerebrovascular diseases (r=0.494,0.454,P<0.01). Both genotypes and allele frequencies of-13T/C at the 5’-flanking region have no significant differences in the two groups (P> 0.05). The polymorphisms of -13T/C has no association with ischemic cerebrovascular diseases.ConclusionsThe data suggest that as an inflammatory marker of atherosclerosis, SAA paricpates in the occurrence and development of ischemic cerebrovascular disease, but if can SAA be an independent risk factor of ischemic cerebrovascular disease or not should be confirmed by a large number of clinical and experimental studies. Its -13T/C polymorphism may be no association with ischemic cerebrovascular diseases, but other sites’has yet to be verified.

Related Dissertations

  1. Study on the Single Nucleotide Polymorphisms in Patients with High Myopia Using SnaPshot,R778.11
  2. Molecular Genetic Analyses of a Family with Congenital Cataract,R776.1
  3. Structural and HblMYC1 Gene Correlated with Natural Rubber Yield in Hevea Brasilensis Muell. Arg.,S794.1
  4. Different host plants chestnut gall wasp mtDNA CO Ⅰ and CO Ⅱ gene sequences and phylogenetic analysis of the relationship,S763.3
  5. Studies on Genetic Polymorphism and Spatio-temporal Dynamics of Puccinia Striiformis f. sp. Tritici in the South of Gansu Province,S435.121
  6. Minority Yi HLA Ⅱ genes DQB1 genotyping methods and gene polymorphism,Q987
  7. Association of Genetic Susceptibility To Lead Toxicity with Single Nucleotide Polymorphism in XRCC3 and XPD Genes,R131
  8. Connecting Research to Liver X Receptor α Gene Polymorphisms-115A and -6A of the Patients with Coronary Heart Disease,R541.4
  9. Study on the Effects of Different Types of Helicobacter Pylori on Expressions of Cx32, Cx43 and GJIC Function in GES-1 Cells,R57
  10. Relationship Between the Blood Concentration of Calcineurin Inhibitors, Single Nucleotide Polymorphisms of Diabetes Mellitus and Posttransplantation Diabetes Mellitus in Renal Transplantation Patients,R587.1
  11. Relationship Between the Intima Media Thickness of Common Carotid Artery (c-imt)and Adiponectin Level and Gene Polymorphism in Promoter Region in Patients with Metabolic Syndrome Accompanied with Type 2 Diabetes Mellitus,R587.1
  12. mtSNP 10398A / G and differences in Hunan Han women groups the risk of ovarian and cervical cancer Correlation,R737.3
  13. Correlation of Exon 4、6 and 10 Single Nucleotide Polymorphisms of GHR Gene with Idiopathic Short Stature,R725.9
  14. The Association Study of DNA Double-strand Break Repair Genes BRCA1、BRCA2 and NBS1 Polymorphisms with Susceptibility to Molecular Epidemiology of Lung Cancer,R734.2
  15. Relationship between the Gene Polymorphism of CETP/TaqIB and Aerobic Performance,G804.2
  16. Study on the Genetic Effect of CAPN1 Gene on Muscle Tenderness in Chicken and It’s Expression Characterization,S831
  17. Genetic Effect and Expression Characterization of Candidate Genes of Fatty Acid Binding Protein in Chicken,S831
  18. Molecular Cloning, Tissue Expression and Polymorphism Analysis of Cav-1,3 Genes in Duck,S834
  19. The Correlation between Crohn’s Disease and the Expression of IL-32 and Gene Polymorphisms,R574.62
  20. Association Study Between Interleukin-1 Gene Cluster Single Nucleotide Polymorphism with as in Anhui Han Population,R593.23
  21. The Study on Association of Cytokine Polymorphism of Immunity with Invasive Fungal Infection in Hematological Patients,R519

CLC: > Medicine, health > Neurology and psychiatry > Neurology > Cerebrovascular disease > Acute cerebrovascular disease ( stroke)
© 2012 www.DissertationTopic.Net  Mobile