Dissertation > Excellent graduate degree dissertation topics show

4-Amino-1,8-naphthalimide Based Fluorescent Probes for ROS: Design, Synthesis and Property

Author: SunXiaoLong
Tutor: QianXuHong
School: East China University of Science and Technology
Course: Applied Chemistry
Keywords: Molecular probe 1,8-Naphthalimide Copper ion Nitric oxide Peroxyl radical
CLC: O621.2
Type: Master's thesis
Year: 2012
Downloads: 144
Quote: 0
Read: Download Dissertation

Abstract


In the work, two different fluorescent turn-on probes (FS, Cu2+[FL]), based on 4-amino-1,8-naphthalimide chromophore, were designed and synthesized successfully. All the compounds were characterized by’H NMR, 13C NMR and HRMS. Their photophysical properties and responses to various ROS were investigated by means of UV-Vis and fluorescent methods systematically.1. A new o-phenylenediamine-based fluorescent molecule FS was synthesized as chromogenic and fluorogenic NO probe using Cu2+ as a promoter. The Cu2+[FS] generated through combining FS with Cu2+ exhibited a significant fluorescent increase and a large blue shift of 200 nm upon addition of nitric oxide in neutral buffer solution. The fluorescence response toward nitric oxide is highly selective and sensitive over other biologically ROS and RNS. The analytical detection limit (ADL) was 30 nM. It is a novel copper-promoted nitric oxide fluorescent probe based on the o-phenylenediamine and opens up new possibilities for the construction of colorimetric and fluorescent sensor for nitric oxide.2. Copper complex-based probe Cu2+ [FL] was designed and synthesized through combining compound FL with Cu2+. The probe showed weak fluorescence due to the paramagnetic quenching from Cu2+. AAPH (2,2’-azobis-2-methyl-propanimidamide, dihydrochloride) is a water-soluble azo compound which is used extensively as peroxyl radical (ROO) generator. The probe Cu2+[FL] demonstrated a 46 nm blue-shift in absorption (from 514 nm to 468 nm) with a large colorimetric response and an off-on fluorescent response upon addition of AAPH (ROO) in neutral buffer solution. The fluorescence response toward ROO is selective and sensitive over other biologically ROS and RNS. To the best of our knowledge, this is the first copper complex fluorescent probe for peroxyl radical(ROO).

Related Dissertations

  1. Studies on the Effects of Exogenous Nitric Oxide on Rice under Cadmium Stress,S511
  2. Changes and Roles of Plasma Hydrogen Sulfide in Elderly Patients with Ischemic Heart Failure,R541.6
  3. The Relationship of Serum Adiponectin、Nitric Oxide Synthase and Carotid Atherosclerosis in Patients with Acute Cerebral Infarction,R743.33
  4. CHD patients with depression nitric oxide synthase gene G894T polymorphism,R749.4
  5. Detection and Clinical Study of Stem Cell Factor in Serum of Acute Asthmatic Patients,R562.25
  6. Study Relationship between TCM Differentiation Syndromes of Coronary Heart Disease Patients and Serum NO and Plasma ET,R259
  7. Amlodipine on LDL oxidation induced bone marrow progenitor cells injury,R965
  8. After vagotomy mouse islet insulin , Bax protein expression and nNOS immunoreactive nerve endings distribution changes,R587.1
  9. Influence of Estradiol on Macrophage Phagocytosis Function and Its Mechanism Study,R543
  10. Study on the Protective Effect of Pae on HUVECs Injured by Hyperlipidemic Serum and Explore the Molecular Mechanism Further,R285.5
  11. Study on Prophylaxis of Sinusoidal Obstruction Syndrome by Ginsenoside Rb1 in Rat Model and Its Signal Transduction Mechanism,R285.5
  12. The Study on the Treatment of Chinese Herbal Medicine-Yizhikang Powders to Dairy Cattle with Retained Placenta,S858.23
  13. The Study of Catalytic Diesel Soot Oxidation Performance on MnOx-CeO2 Complex Oxides,X511
  14. The Effect of No on Marine Phytoplankton Growth and the Carbon Dioxide System,Q945
  15. The Preliminary Study on the Relationship of NO and the Salt-stress Physiology of Dunaliella Salina,Q945.78
  16. Soft Sensing of Cuprum Ion Concentration Based on Machine-Vision for Extraction Process,TP274
  17. Research on the Mechanism of Stomach Intestine Dynamia and Visceral Sensation of Zhongyao on IBS-D Rats,R285.5
  18. Anti-inflammatory Effects of Pentamethylquercetin in LPS-induced Mouse RAW264.7 Macrophages,R285.5
  19. Establishment of Experimental Rabbit Mastitis Model and Effects of Rabbit Mastitis on Expression of Anti-oxidant Index and Content of Acute Phase Proteins,S858.23
  20. The Effect of Chaishao Chengqi Decoction on Nitric Oxide Synthase Neurons of Intestinal Tract in Rats with Severe Acute Pancreatitis,R285.5
  21. Needle knife third lumbar transverse process syndrome model rat central CCK-8, NOS impact study,R245

CLC: > Mathematical sciences and chemical > Chemistry > Organic Chemistry > Organic Chemistry general issues > Properties of organic compounds
© 2012 www.DissertationTopic.Net  Mobile