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Human MGC5306 prokaryotic , eukaryotic expression vectors and positioning of subcellular structures

Author: Wang
Tutor: LeiMing
School: Northwest University of Science and Technology
Course: Biochemistry and Molecular Biology
Keywords: MGC5306 Prokaryotic / eukaryotic expression Fusion protein Subcellular structures Colocalization
CLC: Q78
Type: Master's thesis
Year: 2008
Downloads: 17
Quote: 0
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Abstract


MGC5306 gene as a newly discovered gene , at home and abroad for their research -related reports is still very small. There is experimental evidence that MGC5306 involved in ovarian cancer . MGC5306 function and its mechanism is not yet fully understood. We use technology to build a MGC5306 recombinant prokaryotic expression system, and successfully expressed MGC5306 (98-204AA). We constructed pDsRed-MGC5306, and transfected into Hela cells , subcellular structures observed distribution and positioning within the nucleus . The DsRed-MGC5306 and GFP-SUMO1 were transfected into Hela cells by confocal found that they were located in the nucleus .

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CLC: > Biological Sciences > Molecular Biology > Genetic engineering (genetic engineering)
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