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Adriamycin - folic acid nanoparticles tongue carcinoma Notchl mRNA, WT-P53mRNA expression

Author: GaoZuoZuo
Tutor: JinXiaoMing
School: Qingdao University
Course: Clinical Stomatology
Keywords: Doxorubicin - folic acid nanoparticles Tca8113 tongue cancer cells microcosmic structure Notch-1mRNA WT-P53mRNA
CLC: R739.86
Type: Master's thesis
Year: 2011
Downloads: 28
Quote: 0
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Abstract


[Objective] To determine Adriamycin-folic acid nanoparticles affect on the expression and micro structure of Notch-1 mRNA and WT-P53mRNA in tongue cancer cells,and to probe discuss its anti-cancer mechanism.[Methods] Tongue cancer cells in vitro Tca8113 was respectively treated by means of adriamycin group, folic acid nanometer particle group, adriamycin-folic acid nanoparticle group, and established the control group culture cell, using RT-PCR to detect Notch-1 mRNA and WT-P53mRNA expression changes in each group,the structure changes of each group were detected by the transmission electron microscopic(TEM).[Results]RT-PCR shows that WT-P53mRNA expression level of folic acid nano doxorubicin particles intervention group higher than that of doxorubicin group、folic acid nanometer particle group、the control group (P<0.05), Notch-1 mRNA expression level below doxorubicin group、folic acid nanometer particle group、the control group (P<0.05), There have significantly difference in the expression level of WT-P53mRNA, Notch-1 mRNA between folic acid nano doxorubicin particles intervention group and doxorubicin group、folic acid nanometer particle group、the control group. the expression level of Notch-1 mRNA and WT-P53mRNA between folic acid nanoparticles group and control group have no difference (P>0.05). The lost polygonal structure of cells in acid nano doxorubicin particle group and doxorubicin group is scanned by TEM, TEM shows that mitochondria is swelled, endoplasmic reticulum is degranulated, ribosomes is decreased, a few apoptotic body is observed, there is obvious change in folic acid nanometer particle group.[Conclusion] Doxorubicin-folic acid nanoparticles may induce cell apoptosis and the microcosmic structure change by down-regulate Notch-1 mRNA expression and reduce WT-P53mRNA expression, give play to inhibit proliferation function of Tca8113 tongue cancer cells, its effect is better than that of free doxorubicin.

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CLC: > Medicine, health > Oncology > Oral cavity, maxillofacial tumors > Tongue cancer, sublingual tumor
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