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In this study, based on traditional Chinese medicine theory of pharmacology and Veterinary Pharmacology, TMP and Chinese medicines associated with antimicrobial synergy dose-effect relationship and its impact on immune function associated with research, developed for TMP and traditional Chinese medicine antibacterial compound preparation The scientific basis for the theoretical basis for TMP with traditional Chinese medicine combined with prevention and treatment of poultry diseases. The test tube double dilution method TMP single herb of the minimum inhibitory concentration (MIC) of Staphylococcus aureus, Escherichia coli and Salmonella. Herbs skullcap, Qin skin, Pulsatilla flavescens, according to the MIC values ??to set the high dose, middle dose, low dose level, orthogonal design L9 (34) 9 group side, and then on the square In vitro antibacterial activity is measured and determined to be the optimal combination of traditional Chinese medicine; using the pour plate count method for the determination of the associated TMP and traditional Chinese medicine used as the three bacteria 1h, 2h, 4h and 8h after the number of bacteria, and calculate the sterilization rate with a minimum -squares fitting, sterilization rate calculated TMP optimal amount of additive; composed of traditional Chinese medicine on the basis of drug combinations, again vivo antibacterial activity of the herbal compound mixture, and finally organ coefficient of determination, peritoneal macrophages swallowed a chicken erythrocytes turbidimetry and MTT assay, respectively peritoneal macrophage phagocytosis of immune organs index, serum lysozyme activity, the level of serum hemolysin, lymphocyte proliferation and hematological indices were measured to detect traditional Chinese medicine mixture on immune function in mice. The test results showed that: 14 Coptis, forsythia, skullcap herb have antibacterial activity, which Coptis the strongest antibacterial activity, MIC value order 0.016,0.016 and 0.031 g of the mL-1; the forsythia followed, MIC values ??were 0.031g · mL-1. Prescription herbal compound (the skullcap, Qin skin, Sophora and Pulsatilla compatibility ratio 1:4:2:1), the MIC values ??of three pathogens are 0.125g · mL-1. Calculate the the TMP optimal amount of additive Staphylococcus aureus, Escherichia coli, The different Salmonella: the Coptis 58.3,55.8,28.5 mg · g -1 ; honeysuckle 3.86,1.98,3.95 mg · g -1 ; the Sophora flavescens 3.62,2.12,2.20 mg · g -1 ; forsythia 7.1,7.3,7.1 mg · g -1 ; dandelion 4.0,1.8,2.0 mg · g -1 ; Qinpi 7.62,4.62,3.98 mg · g -1 ; Pulsatilla 4.01,1.85 , 2.10mg · g -1 ; the skullcap 27.25,13.81,7.14 mg · g -1 ; herbal compound 1.94,1.94,1.98 mg · g , - 1 . In vivo antibacterial prevention and treatment trial, the high dose of Chinese medicine drug combinations have a preventive effect on more than 90% of the infected mice and of mice mortality rate to below 20%. Dose and high dose of the herbal compound agent can significantly increase the immune organ index, peritoneal macrophage phagocytosis and serum lysozyme content (P lt; 0.05), significantly increase the normal mouse erythrocyte total number, the total number of white blood cells and hemoglobin content ( P lt; 0.05), to promote the role of non-specific immune function of mice; while also significantly improve the level of serum hemolysin and lymphocyte proliferation (P lt; 0.05), to enhance the specificity of the humoral and cellular immune immune function.
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