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Study on the Genetic Diversity of 9 Chinese Indigenous Goat Breeds Using Microsatellite Analysis

Author: FanZuo
Tutor: LuoJun；LiuChouSheng
School: Northwest University of Science and Technology
Course: Genetics
Keywords: Local goat breeds in China Microsatellite Genetic Diversity Population structure
CLC: S827
Type: Master's thesis
Year: 2009
Downloads: 37
Quote: 1
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Abstract


This study nine local goat breeds (Henan corbels, Guangfeng, Jiangxi West, Ma Tau, Yichang white Xiangdong black, Fuqing Dai Yun and Chuandong white goat) and one introduced species (Boer goat) a total of 563 individuals as the object of study the joint recommendation of the UN Food and Agriculture (FAO) and the International Society for Animal Genetics (ISAG) 16 microsatellite primer ABI3100-Avant automatic gene sequence analyzer platform combined fluorescence - multiplex PCR technical analysis of the genetic diversity of the 10 goat breeds, provide the basis for the rational use of resources and protection of our local goat genetic, experimental results are as follows: 1.16 microsatellite loci detected 162 in the 10 varieties bits greater than 4, so the number of alleles of the genes, of which 15 microsatellite loci using 15 microsatellite loci were analyzed. The maximum number of alleles DRBP1, 16 alleles were detected, ETH10 and SRCRSP3 detected at least six alleles. Different distribution pattern of the same alleles of microsatellite loci in different varieties. Hardy - Weinberg equilibrium test: each species on 2-8 sites deviate from Hardy - Weinberg equilibrium (P lt; 0.05), 15 microsatellite loci, only DRBP1 all varieties deviate from Hardy - Weinberg equilibrium. 3.15 microsatellite loci in 10 goat populations, in addition to ETH10 microsatellite loci in two varieties of the Fuqing goats and Daiyun goat polymorphism of 14 microsatellite loci in the nine local varieties and introduced varieties showed polymorphism. 10 goat populations polymorphic information content (PIC) 0.4429-0.6442 between observed heterozygosity range between 0.3732-0.6442, expected heterozygosity range between 0.5040-0.6890. 4.F-statistical test results show: 9 local goat breeds in the 15 sites on the sub-population fixation index (Fis) an average of 0.113, reflecting a nine goat populations there is a certain degree of inbreeding. The Fst average of 0.105, indicating that 89.5% of the genetic variation exists in breeds, while 10.5% of the genetic variation existed between varieties. Gene flow (Nm) between groups in the 0.878-20.583 between changes. 5. Phylogenetic tree based on Nei's standard genetic distance (DS) and the DA genetic distance the UPGMA cluster NJ law clustering, the results show that the diagram (DA, UPGMA) more reliable. Matou goat and Yichang white goats first clustered into one group, then Chuandong white goat join, the goat of Ganxi Xiangdong black goat as a class to join, and then the Henan the corbel goats and Guangfeng goats are then added, of Fuqing goat and wearing cloud goats together as another type of join. Finally, nine local goat breeds together with the Boer goat. The Structure software 's analysis of the population structure of the 10 goat breeds, can be divided into four groups. The same dendrogram group relations results are basically the same.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Livestock > Goat
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