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Effects of Barking on Physiological Characteristics and Contents of Secondary Metabolites during Growth of Eucommia Ulmoides Oliver
Author: ChengNa
Tutor: DongJuanE
School: Northwest University of Science and Technology
Course: Forest Products Chemical Processing Engineering
Keywords: Skinning Eucommia Physiological characteristics Secondary metabolites Protective enzymes Osmoregulation substances
CLC: S567.19
Type: Master's thesis
Year: 2009
Downloads: 30
Quote: 0
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Abstract
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The Eucommia Is our precious medicinal species, its peeling has been the traditional technique, the physiological mechanisms skinned studied lack; Eucommia skin as herbal medicines, its material basis for the treatment of diseases which contains secondary metabolites. Growth on the the different skinning amount eucommia; the eucommia vivo SOD, POD, CAT protection activity, soluble sugar, soluble protein and free proline Osmotic Adjustment Substances PAL activity and secondary metabolite contents change The study made the following conclusions: 1. the peeling treatment Eucommia ground diameter, trunk diameter and a new branch is still growing, but the overall growth than the control strain. Rough growth of 50% vs 75% and 100% skinned amount of processing plants tree faster, but the difference is not peeling with 75% and 100% of the amount of processing. Each peeling down the new sticks growth amount is growing, but the growth rate is smaller than the control strain, processing plants skinned amount of 100% new branch growth compared with 50% and 75% of large processing strains. Skinned and ground diameter growth and diameter growth of the analysis showed that showed a significant negative correlation. Skinning the larger the amount, the greater the impact on tree growth, but peeling will not Du Zhongsheng long stop. The variance analysis showed that 75% of its peeling treatments had no major impact on the rough tree growth and the growth of new skin, tree faster recovery. Therefore, in the actual production of the best 75% peeling to get the best value for money, and protect Eucommia resources. With peeling increase in the amount of Eucommia vivo SOD, CAT and POD protect enzyme activity showed a tendency to increase, which a skinned amount of 100% protection of enzyme activity with other treatment differences significant, 75% and 50% the peeling treatment amount of protection enzyme activity can be quickly adjusted to a level consistent with the control strain. These results suggest that mechanical injury (stress) disorder, causes the organism metabolic processes Eucommia tissue cells to form a number of physiological and biochemical adaptation mechanisms. Eucommia body of SOD, CAT and POD three protective enzymes in varying degrees of stripping the amount of processing rapidly start synergies effectively prevent Skinning Eucommia tree caused by lipid peroxidation damage Eucommia enhance the comprehensive resistance, to ensure the normal growth of the tree. 3. PAL plants subject to environmental stress resulting change another protective enzymes, SOD, POD and CAT protection mechanisms. The plant hurt, Another defense system (secondary metabolites) is open. The results of this study show, the Eucommia peeled tree vivo PAL activity than the control plant height, and the difference was significant. Therefore, PAL rapid accumulation of Eucommia by peeling mechanical injury, from the other hand played a protective role of Eucommia tree. 4 peeled the Eucommia soluble sugar content changes to resist peeling Eucommia tree caused damage, the greater the amount of peeling, soluble sugar content greater the change in each treatment to recover to the level of the control strain in a relatively short period; each compared to the peeling process Eucommia control strains in the soluble protein content of the growth period no significant changes, but re-grow new skin due to the low degree of aging, high protein synthesis capacity, making the soluble protein content in the final tree growth than control strain; the different peeling treatment conditions eucommia free proline were higher than in the control strain, which 100% of the highest peeling treatment, 50% and 75% treatment difference was not significant. The accumulation of free proline body Eucommia tree Since skinned osmotic pressure caused a response. The amount of processing conditions by peeling acid content of chlorogenic acid and geniposide with peeling amount increases upward trend, and there are significant differences with the control strain, indicating skinned stimulate the the Eucommia body of chlorogenic acid and genipin nucleotide synthesis. Total flavonoids content with peeling increase in a significant increase in a significant difference between 100% skinning amount of total flavonoids and control strain. Aucubin content consistent regularity in the growth period after peeling. These results suggest that appropriate Skinning (mechanical stimulation) can be caused by the eucommia tree vivo chlorogenic acid, geniposide acid, aucubin and total flavonoids content of secondary metabolites changes, different kinds of secondary metabolite contents with peeling The amount of the variation is different.
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