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In this study, leaf tobacco (Nicotiana glutinosa) and ordinary cigarette K326 (Nicotiana tabacum var.K326) as host materials to tobacco mosaic virus (Tobacco mosaic tobamovirus, TMV) as tested drug sources, measured Walnut petroleum ether extract phase anti-TMV activity and its mechanism of action has been studied mainly the following results: (1) using active tracking means of Walnut petroleum ether phase anti-tobacco mosaic virus isolated active ingredient tracking. This phase was gradient to give a high active ingredient A9, A9 in the inoculum 10mg.mL-1, the leaf tobacco to TMV infection inhibition rate was 71.00%. The A6-phase chromatographic fractions isolated and purified by repeated chromatography to give compound 1, the initial determination is lignin compounds, its structure is: (2) analysis using the dry spots were determined A9 heart leaf smoke a blunt on TMV of the role of passivation 0.5h, 1h and 2h after inoculation concentration 10mg.mL-1, the inhibition rates were 26.73%, 54.01% and 32.64%. Description A9 to TMV with in vitro inactivation, 1h after its inactivation strongest. (3) Method for Determination of necrotic lesions heart leaf tobacco on the A9 initial infection to TMV TMV replication inhibition and inhibition of proliferation. The results show that, A9 has the role of primary infection prevention TMV TMV replication and inhibition of proliferation and inhibition of primary infection to TMV stronger than inhibiting proliferation of TMV replication. First spraying inoculation, the inhibition rate was 52.52% after 24h than 48h after the first spraying after inoculation, the inhibition rate than after 48h 24h, as 31.17%. (4) A9 prevention and treatment of post-processing ordinary cigarette K326 3d, 6d, 9d and 12d, the measured chlorophyll, malondialdehyde (MDA), flavonoids, total phenols, soluble protein content and polyphenol oxidase ( PPO) and peroxidase (POD) activity, the results showed that after treatment A9 prevention and treatment of K326 leaves chlorophyll, flavonoids, phenols and total soluble protein content increased, MDA content decreased, POD and PPO activity was enhanced, Visible, Walnut low polar components can induce plants produce disease resistance, and enhance resistance of tobacco to TMV infection. (5) ultrastructural observations show: After A9 prevention and treatment after treatment, ordinary cigarettes, significantly reduced intracellular TMV particles, and of varying lengths, prevention is better than treatment treatment treatment; infection after TMV tobacco film most common cell structure subject to different degrees of damage, from the A9 TMV in tobacco leaves treated mitochondria fracture, the disintegration of chloroplasts and mitochondria is mitigated, and produce large osmiophilic particles and membrane body.
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