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Purpose in our country, the incidence of colorectal cancer increased year by year, has been ranked fourth in the malignant tumor. Early colorectal cancer often no obvious symptoms, treatment Shique 15% to 35% of patients have been transferred, in which the liver is the most common and the most important sites of metastases in patients with advanced liver metastases incidence is as high as about 50% to 70%. Currently, the treatment of colorectal liver metastases: surgery, chemotherapy, radiotherapy and molecular targeted therapy, but once liver metastasis, the patient survival rate was significantly lower, single or confined to a leaf in a median survival of less than 24 months, less two leaves were transferred, the median survival time of less than 18 months, if left untreated, most patients survive for a period not more than 9 to 12 months. Therefore, in patients with colorectal cancer, such as prediction or early detection of liver metastases, to give timely and appropriate treatment to prolong survival is important. With the development of molecular biology, the study found that a variety of genes and their products may be involved in the invasion and metastasis of colorectal. Regenerating liver phosphatase -3 (Phosphatase of regenerating liver cell in-3, PRL-3) is a recently discovered a protein tyrosine phosphatase (Protein tyrosine phosphatase, PTP), numerous studies show that with colorectal liver metastasis related. Meanwhile, angiogenesis and tumor invasion and metastasis formation of numerous blood vessels associated factor, vascular endothelial growth factor (Vascular endothelial growth factor, VEGF) is by far the most important angiogenic factors. The purpose of this study is to investigate the expression of PRL-3 and VEGF in colorectal cancer in the primary tumor and its relationship with colorectal liver metastasis, while analysis of patients with general clinical pathological features of colorectal liver metastases relationship. Collected Ningxia Medical University Hospital from 1998 to 2005, complete follow-up data by histopathology confirmed 114 cases of colorectal cancer cases, 63 cases of liver metastases confirmed by means of imaging (B ultrasound, abdominal CT, MRI) and other tests, and the exclusion of primary liver tumors (36 cases and liver metastases, 27 cases of metachronous liver metastases), 51 patients were followed up for at least three years found no recurrence and metastasis. Full set of 71 cases of males, 43 females, aged from 34 to 82 years, with a median age of 57 years old; 102 cases of the Han, Hui 12 cases; tumor located in the rectum in 74 cases, sigmoid 19 cases, 11 cases of ascending colon, descending colon seven cases one cases of transverse colon, ileocecal two cases; according to the 2002 AJCC staging of colorectal cancer, surgery, pathology staging: Ⅰ 16 cases, 45 cases, Ⅱ, Ⅲ of 17 cases, Ⅳ 36 cases; pathological types: 110 cases of tubular adenocarcinoma (poorly differentiated 15 cases, in differentiation 95 cases), 2 cases of mucinous adenocarcinoma, small cell carcinoma and papillary adenocarcinoma in 1 case; tumor size in diameter 4cm for the sector were divided into two groups, ≤ 4cm in 52 cases, gt; 4cm 62 cases; 51 patients with ACS.180SE automatic chemiluminescence analyzer detection of serum CEA, CA199 values. Due to inadequate funding experimental systematic sampling methods drawn from the 114 cases 69 patients using immunohistochemical method detected 69 cases of colorectal carcinoma, 69 cases of cancer and adjacent normal colorectal tissue and 28 cases of benign colorectal tissues PRL-3 VEGF expression analysis of both expression in colorectal cancer. 28 cases of patients with benign lesions from healthy individuals. The experimental results were statistically analyzed using SPSS13.0 software. 1, PRL-3 expression in colorectal cancer was significantly higher than in adjacent normal colorectal tissues, the positive expression located in the cytoplasm; PRL-3 expression and tumor depth of invasion, and lymph nodes, liver metastasis and patient survival nothing to do. 2, VEGF expression in colorectal cancer tissue than in adjacent normal colorectal tissue, its positive expression localized to the cell cytoplasm, and the depth of tumor invasion, lymph node metastasis and liver metastasis is closely related to the survival of patients with positive expression was significantly lower than the negative expression persons. 3, PRL-3, VEGF in patients with liver metastases rate are positive group was significantly higher than the one for the positive and two were negative group. 4, liver metastases of colorectal cancer patients course of the disease, tumor depth of invasion, lymph node metastasis, serum CEA, CA199 values, regardless of the patient's age, gender, ethnicity and whether the line of postoperative adjuvant chemotherapy. 6, Logistic regression analysis showed: the depth of tumor invasion, lymph node metastasis, the expression levels of VEGF in cancer tissue and in patients with disease duration were independent risk factors for colorectal liver metastases. 5, the survival rate of patients with liver metastasis was significantly lower than the liver metastasis group survival in patients with liver metastases showed a lower trend than metachronous liver metastasis group, but the difference was not statistically significant. Conclusion 1, PRL-3 in colorectal cancer, high expression related with the depth of tumor invasion, metastasis and survival has nothing to do with the liver, prompted PRL-3 is involved in the incidence of colorectal cancer development, but not yet as colorectal cancer liver metastases and prognosis predictor. 2, VEGF in colorectal cancer tissues high expression of its expression and tumor infiltration depth, lymph node metastasis, liver metastasis and patients survival is closely related to suggest that VEGF involved in the colorectal cancer occurrence, development and liver metastasis, can become the judge of colorectal liver metastasis and predictors of prognosis. 3, PRL-3, VEGF in patients with liver metastases rate are positive group was significantly increased, suggesting that the combined detection of PRL-3 and VEGF may be more certain guiding significance in predicting colorectal liver metastasis. 4, liver metastases of colorectal cancer patients course of the disease, tumor depth of invasion, lymph node metastasis, the expression levels of VEGF in serum CEA, CA199 values, cancer organizations, and the depth of tumor invasion, lymph node metastasis, the expression levels of VEGF in cancer tissue and in patients with disease duration can independently affect colorectal liver metastases.
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