Dissertation > Excellent graduate degree dissertation topics show

Identification and Analysis of miRNA and Flower Development Specific Genes in Wheat

Author: WeiZhaoYan
Tutor: MaZhengQiang
School: Nanjing Agricultural College
Course: Biochemistry and Molecular Biology
Keywords: EST Gene expression profile Flower MicroRNA Target
CLC: S512.1
Type: Master's thesis
Year: 2009
Downloads: 20
Quote: 0
Read: Download Dissertation

Abstract


Large-scale cDNA sequencing produced millions of ESTs representing differentiated biochemical and functional states in different tissues. EST analysis can be used for gene discovery and gene expression profiling. Genes specifically expressed in wheat flower were identified using a computer-based strategy in this study.153 contigs specifically expressed in flower were identified from 77657 wheat contigs. Specific expression of 11 genes was validated by the result of RT-PCR. Functional annotations showed these gene specifically expressed in flower are related to several pathways such as alkane biosynthesis, flavonoid biosynthesis and ethylene biosynthesis.MicroRNAs are a class of endogenous small non-coding RNAs about 20-24nt long which play an important role in the development of plant. Using previously known miRNAs from plant in the miRBase database against EST and GSS databases, we identified 41 potential miRNAs in wheat. Potential targets except miR395 and miR399 were predicted by searching EST databases, most of which are transcription factor. Five wheat miRNAs were analyzed by RT-PCR, and the expression of four was tissue specificity.

Related Dissertations

  1. The Key Technologies of Autocollimation System Based on the Beam-Splitting Target Detecting Feedback of Laser Beam Shift,TH741.14
  2. Maneuver Detection and Tracking with the Radar Rate Measurement,TN953
  3. The Maneuvering Target Tracking Research Based on VRPF,TN957.52
  4. ISAR Imaging Simulation of Space Targets and Target Recognition Based on ISAR Images,TN957.52
  5. Research on Multi-Sensor Embattling and Continious Tracking Technique Applying for Anti-Stealth and Anti-Interference,TN953
  6. The Research and Implementation of Software Oriented Fault Injection Tool,TP311.52
  7. Research on Joint Target Detection for Dual-Sensor Image and System Implementation,TP391.41
  8. Study on Virtual Detector of Infrared Hyper-Spectral Image,TP391.41
  9. Research on the Image Real-Time Acquisition, Storage and Image Processing System,TP391.41
  10. Study on Nonlinear Variable Structure Guidance Law,TJ765
  11. The Research of Guidance and Control Method in Terminal Guidance Phase,TJ765.3
  12. Research and Application of Diverse Density Learning Algorithm,TP181
  13. Research on Target Tracking and System in Wireless Sensor Networks Based on Character of Movement and Terrain Restriction,TP212.9
  14. Application and Research of Dual Control in Vertical Landing Aircraft,TP273
  15. Development of EST-SSR Primers and Application in Analysis of Genetic Realtionships in Tree Peony,S685.11
  16. Process Optimization and Degradation Kinetics for Microwave Vacuum Drying of Different Traditional Chinese Medicine Extract,TQ461
  17. Study on Tolerance of Five Species of Garden Flower to Copper Stress,S68
  18. Comprehensive Quality Assessment of College Students,G645.5
  19. Marketing Strategy of China campus buy,F274
  20. Gene Prediction and Sequence Analysis of Insect OBP CSP and sid-1,Q78
  21. SRAP and SSR molecular markers of pink flower Dendrobium genetic diversity and population genetic structure,S567.239

CLC: > Agricultural Sciences > Crop > Cereal crops > Wheat > Wheat
© 2012 www.DissertationTopic.Net  Mobile