Dissertation > Excellent graduate degree dissertation topics show

Determination Method and Extraction Purification of Terpenoid Indole Alkaloids in Catharanthus Roseus

Author: ZhangJing
Tutor: LiuZhaoPu
School: Nanjing Agricultural College
Course: Marine biology
Keywords: RP-HPLC Vindoline Vincristhine Catharanthine Vinblastine Serpentine
CLC: R284
Type: Master's thesis
Year: 2010
Downloads: 19
Quote: 0
Read: Download Dissertation


Catharanthus roseus (L.) G. Don is one of the Catharanthus in Apocynaceae. It widely grows in south of the Yangtze River of China. There are lots of alkaloids in it, of which important anti-tumor terpenoid indole alkaloid (TIAs) has abroad-spectrum anticancer activity, it can treat much kinds of cancer. The detection and purification of catharanthus various alkaloids have an important theoretical and practical significance on industrial production in Vinca.This experiment used RP-HPLC, ultrasonic-methanol extraction and resin purification, established the analytical methods of five kinds indole alkaloids in roseus and sample preparation method we established RP-HPLC determination method and sample preparation method, and study on optimizing the extraction process of four important alkaloids from Catharanthus roseus, and finally we established the new vinblastine purification process by macroeticular resins. Here are the main conclusions:The HPLC detection method of five vinca alkaloids:Vindoline, Vincristhine, Catharanthine, Vinblastine, Serpentine was established. These five ingredients were separated on mobile phase:250 mL water-triethylamine (990:10, pH adjusted with phosphoric acid is 7.2) mixture of 750 mL methanol-acetonitrile (400:100); The flow rate was 0.8 mL/min, sample load set at 20μL; detection wave length was set at 220 nm, column temperature was set at 21℃.The correlation efficients were 0.9998~0.9985 in the linear range. The RSD of themethodwas less than 3%. The average recoveries ranged from 96.4% to 98.8%. Five indole alkaloids can completely separate well in 22 min. The method is simple, rapid and accurate.The extraction method and comprehensive optimization of condition in ultrasonic-methanol method were studied, the extraction conditions are as follows:Methanol as solvent was used for extraction 150 min, which pH value was 4.0. The ratio of mass of raw material to the volume of solvent is 1:15, and extraction temperature is 45℃. The vindoline and catharanthine extraction obtained by such optimization were 0.164% and 0.081% respectively.The macroporous resin and purification process of Vinblastine method:the sample compared to the macroeticular resins= 5:1, the sample concentration is 0.5 mg/mL, eluate of 95% ethanol solution, the elution volume is 25 ml/g of the unsaturated resin, resin regeneration conditions are:first, the concentration of 5% hydrochloric acid leaching, and then the concentration of 3% sodium hydroxide wash to neutral, and then soaked in ethanol, the final wash with plenty of distilled water to the non-alcoholic flavor. Resin can be recycled four times, through the regenerated resin adsorption effects can be achieved of the original resin adsorption capacity of over 90%.

Related Dissertations

  1. RP-HPLC Method for Lipophilicity Measurement of Compounds,O656.2
  2. The Purification and Isolation of Antimicrobial Peptides from the Grass Carp Intestine and the Research on Physicochemical Properties,S917.4
  3. Purification and Analgesic Activity of CTXn from Naja Naja Atra Venom,R96
  4. RP-HPLC of different cyclodextrin derivatives with cilostazol inclusion studies,R914
  5. Preliminary Study on Anti-tumor Effect of Sinapine on H22 Mice Hepatocellular Carcinoma and Its Mechanisms,R735.7
  6. Establishment of Quality Standard and Fingerprint Chromatograph of Ipomoea Batatas (L.) Lam. Simon No.1,R284
  7. Study on Antioxidative Peptide of Peanut Meal,TS229
  8. Research on the Preparation Technology of Magnesium Chloride Hexahydrate and Light Magnesium Oxide from Serpentine,TQ132.2
  9. The Extraction and Purification of Tea Saponin and the Extraction of Tea Seed Polysaccharide in Camellia Cake,S571.1
  10. The Experimentation on a New Saponin for Its Extraction Separation from Homonoia Riparia Lour and the Effects on Hyperuricemia,R965
  11. Studies on Biopharmaceutical Analysis and Preclinical Pharmacokinetics of a Compound Preparation: Amlodipine and Telmisartan,R96
  12. The Study of Rapeseed Protein ACE Inhibitory Preparation and Seperated,TQ464.7
  13. Numerical Study of Finned Serpentine Tubes’ Flow and Heat Transfer Performance in Direct Air Cooling Power Plant,TK261
  14. Direct Air Cooled Condenser air flow and heat transfer characteristics of finned tubes Numerical Study,TK124
  15. Pharmacokinetic and Bioequivalent Studies of Lansoprazole Tablets in Healthy Body,R96
  16. A Pre-Column Derivatization RP-HPLC Method for Determination of Panoxadiol and Panaxatriol in the Cosmetics,TQ658
  17. Studies on the Extraction, Purification and Semi-synthesis of Vinblastine,TQ461
  18. Preliminary Studies on the Biological Functions of One Functional Probiotics Strain,Q93
  19. Analysis of Phenolic Compounds in Foods, Drugs and Cosmetic Products by High Performance Liquid Chromatography,O657.72
  20. Study on Separation and Purification Process of Monoterpenoid Indole Alkaloids Vindoline and Catharanthine from Catharanthus Roseus,R284

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Traditional Chinese medicine chemical
© 2012 www.DissertationTopic.Net  Mobile