Dissertation > Excellent graduate degree dissertation topics show

Study on the Outbreak Mechanism of Soft-rot Disease in Konjac and Its Biocontrol Based on the Resistance Inducement

Author: JiJiaXing
Tutor: YangYing；YuLongJiang
School: Huazhong University of Science and Technology
Course: Biochemistry and Molecular Biology
Keywords: Konjac Soft-rot Temperature Resistence
CLC: S436.32
Type: Master's thesis
Year: 2011
Downloads: 76
Quote: 0
Read: Download Dissertation

Abstract


Konjac (Araceae Amorphophallus) is a perennial economical crop rich in active ingredients including konjac glucomannan which is in urgent need in food, medicine and chemical industry. Thus, konjac is widely cultivated in the southwest of China. However, as the soft-rot disease break out every year explosively and contagiously when the temperature rises dramatically in the summer, this disease has developed into a devastating catastrophe to konjac production. In this work, pathogen responsible for the soft-rot disease in konjac was isolated and identified; the physiological and pathogenic factors, such as growth and pathogenicity, were studied under different temperatures to understand the outbreak mechanism of this disease under evaluated temperatures; 6 externally applied chemical activators to plant defence system were also studied to explore their potentical as soft- rot resistence inducer. The main results were shown as follows:(1) Soft-rot pathogen ES09 was isolated and identified as Pectobacterium carotovorum subsp. carotovorum (Pcc) through 16s rDNA blast and physiological tests.(2) It is suggested that ES09 showed higher growth speed and stonger motility when the temperature was evaluated above 30oC. Denaturing gradient gel electrophoresis (DGGE) revealed, compared to May and November, the population of ES09 in the konjac rhizosphere increased significantly in July.(3) It is suggested that ES09 macerated konjac tuber much stronger under 30oC and 37oC with the pathogenicity test. Excellular enzyme production was strictly regulated under quorum sensing, pectate lyase and polygalactronase was producted in high levels only if the bacteria population met the threshold.(4) The potential of 6 externally applied chemical resistence activators were tested on the konjac leaves cultivated in the lab and plants in field, revealing the infection of ES09 was inhibited in salicylic acid (SA), p-coumaric acid (p-CA), and cinnanamic acid (CA) treated leaves or plants; compared to the control, more excellular H2O2 accumulation and higher peroxidase activity were observed in SA, p-CA and CA treated leaves, suggesting these leaf-spraying chemicals could take effect in the oxidase burst of konjac cells, resisting the attack from the soft-rot pathogen.

Related Dissertations

  1. Preparation and Electrocaloric Effect of BaxSr1-xTiO3 Ferroelectric Thin Films,TB383.2
  2. Research on Temperature Measurement Technique Based on CCD Image Sensor,TH811
  3. Temperature Drift Modeling and Compensation of Fiber-Optic Gyroscopes,V241.5
  4. Simulation Analysis on Temperature Stress of RCC Arch Dam and Its Construction Joints Design Research,TV642.2
  5. Analysis and Study of Abutment Stability in Concrete High Arch Dam by Three-Dimensional Nonlinear Finite Element Method,TV642.4
  6. Research on Nozzle Plume Temperature Distribution Measurement System,V434
  7. Numerical Analysis of Flow Process in a Molten Carbonate Fuel Cell,TM911.4
  8. Research on Temprature Controling Technology of Laser Diode with Thermoelectric Cooler,TN248.4
  9. Research on Temperature Control System for High-Performance Oven-Control Crystal Oscillator (OCXO),TN752
  10. Fly Ash study of low temperature ceramic foam,TU52
  11. Theeffect of Environmental Factor on Rooting Culture of Paeonia Suffruticosa in Vitro,S685.11
  12. Acid solution on the structure and properties of chitosan / hydroxyapatite composite,R318.08
  13. LSGM electrolyte thin films and electrochemical properties of,TM911.4
  14. Research on Integrated Positioning Card Based on ZigBee and GPS Inside/Outside of Tunnel,TN967.1
  15. The Large Concrete Hydration Heat Temperature Effects Research of the Cable Stayed Bridge,U441.5
  16. The Effect of Exercise and Control and Loss Weight on Physical Function in Different Air Humidity Within High Temperature,R87
  17. Numerical Simulation and Strength Analysis in Plug-in and Jet-stream Hot-wind Furnace,TF578
  18. The Study on the Vestibular 3d Reconstruction of Guinea Pig and the Numerical Simulation of Temperature Field,R764
  19. Temperature alkaline secondary smelting of aluminum ash,TF821
  20. Screening of Cold Resistant Agent for Rice in Hunan Province,S511
  21. Effect of Diapause-inducing Temperature and Photoperiod on the Expression of Diapause-related Endocrine Genes in the Silkworm, Bombyx Mori,S881.2

CLC: > Agricultural Sciences > Plant Protection > Pest and Disease Control > Horticultural Crops Pest and Disease Control > Vegetable pests > Yaro class of pests and diseases
© 2012 www.DissertationTopic.Net  Mobile