Dissertation > Excellent graduate degree dissertation topics show

Effects of Berberine on the Rat Carotid Artery Balloon-injury Model and PTEN Expression

Author: HongSheng
Tutor: ZengQiuTang
School: Huazhong University of Science and Technology
Course: Internal Medicine
Keywords: Berberine PTEN Neointimal Vascular smooth-muscle-cell Restenosis
CLC: R285.5
Type: Master's thesis
Year: 2011
Downloads: 12
Quote: 0
Read: Download Dissertation

Abstract


Objective:The goal of this study was to evaluate the effects of berberine on the inhibition of vascular SMC proliferation and carotid artery balloon-injury model rat neointimal formation and restenosis,and explore whether the mechanisms by changing the expression of PTEN achieved.Methods: Application of MTT colorimetric assay to detect the effect of different concentrations of berberine on vascular smooth cell proliferation, RealTime-PCR and Western blot analysis showed that the effects of berberine on the vascular SMC PTENmRNA and PTEN expression at the protein level. Rat carotid artery balloon-injury models were used to investigate the effect of berberine on neointimal formation、restenosis and the expression of PTEN by western blot methods..Results: Berberine inhibited rat aortic smooth muscle cell proliferation. Berberine improves neointimal formation and restenosis(between group B, groupC and groupA was statistically singnificant, between group B and groupC was statistically singnificant,p<0.05). Berberine upregulation of PTEN expression in a concentration -dependent manner. Optimal concentration of berberine is 200μmol/L for the treat of vascular smooth muscle cell. 14 days before the balloon injury and additional 14days after the balloon injury berberine treat better than a simple Postoperative 14days management for restenosis prevention.Conclusions:Berberine inhibits vascular smooth-muscle-cell proliferation and improves neointimal formation and restenosis after rat carotid artery balloon injury model, probably through upregulation of PTEN expression.

Related Dissertations

  1. Study on the Combined Probiotics and Its Effects on Prodution Performance of Pigs,S828.5
  2. Expression and Clinical Significance of PTEN and Caspase-7 in Gastric Cancer and Precancerous Lesion,R735.2
  3. Research of Expression and Clinical Significance of p53、PCNA and PTEN in Gastric Cancer,R735.2
  4. survivin and PTEN in epithelial ovarian tumors and its significance,R737.31
  5. Mechanisms of Inactivation of PETN by Carchnoqen Benzo(a) Pyrene,R730.2
  6. Effects of Berberine on Red Blood Cells of Patients with G6PD Enzyme Deficiency,R55
  7. Impact of Over and Decreased Expression of PTEN on Migration of Human Airway Smooth Muscle Cells,R562.25
  8. Adenovirus-mediated VEGF165 Gene Transfer for Preventing Vein Graft Restenosis,R654.3
  9. The Expression and Significance of DJ-2 and PTEN in Chronic Kidney Disease,R692
  10. The Expression and Clinical Significance of PTEN and PIK3CA in Lung Cancer,R734.2
  11. Studies on the Correlation between Pten、Livin Expression and Chemosensitivity of Esophagfal Carcinoma,R735.1
  12. Study on Correlation between PTEN and PI3K/Akt Pathway in Endometrial Carcinoma,R737.33
  13. PTEN, PI3K and Akt protein expression in ovarian serous carcinoma Correlation of Clinical Pathology,R737.31
  14. Sepsis PTEN expression in endothelial cells,R459.7
  15. Study on the Preparation and Properties of Chitosan-Berberine Composite Membrane,TB383.2
  16. Clinical Research of Polycystic Ovary Syndrome with Insulin Resistance by the Method of Invigorating the Kidney and Regulating Menstruation Cycles Combined with Berberine,R271.9
  17. The Influence of Jiaweihuanglianwendan Decoction on Homocysteine Levels in the Patients with Phl Egm-Heat Stasis Type Unstable Angina Pectoris in Coronary Heart Disease,R285
  18. Cold heat of efficacy of traditional Chinese medicine on the expression and rhubarb , berberine effect on the heat of experimental study in rats,R285
  19. Resources Investigation and Quality Study of Coptis Chinensis Var Brevisepala in Anhui,R282
  20. The Basic and Clinic Research of Resrtenosis After PCI,R541.4
  21. The Inhibitory Effect of Silencing Cx43 Gene by RNAi on Vascular Restenosis,R541.4

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology
© 2012 www.DissertationTopic.Net  Mobile