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Studies on SNPs of DECR1 Gene and Its Relations with Partial Economic Traits in Pigs

Author: MaLe
Tutor: HuangShengQiang
School: Hunan Agricultural University
Course: Animal Genetic Breeding and Reproduction
Keywords: Pig DECR1 gene SNPs Meat quality traits Carcass Traits
CLC: S828
Type: Master's thesis
Year: 2010
Downloads: 23
Quote: 0
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Abstract

In this study, eight pig breeds total of 692 pigs as test material, three exotic breeds (Duroc, Landrace and Large White) and five local pig breeds (Ningxiang pigs, pig Shaziling, Tai Wai sub-pigs Taoyuan pigs, Wuzhishan pig) mitochondrial 2,4 - diene coenzyme A reductase (mitochondrial 2,4-dienoyl-CoA reductase, DECR1) gene SNPs were screened, detected by the gene Exon2 and Exon5 exist There are two polymorphic sites. PCR-SSCP method Exon2 and Exon5 two polymorphic sites of genetic structure analysis, and the correlation of the two polymorphism loci genotypes and meat quality traits and carcass traits were studied. The study concluded as follows: 1, this study, through the use of the public database of information to find the people, cattle, mice and pigs DECR1 CDS sequence of the gene, the the CDS of the above species DECR1 gene sequence homology analysis with DNAStar software homology relationship between human and pig homology of 89.8%. 2, 5 the template design primers to amplify the DECR1 gene sequence pig DECR1 gene exon sequences. PCR-SSCP method of the pig DECR1 gene 5 exon SNPs screening, screening two polymorphisms to Exon2 and Exon5, point results. 8 the pig breeds DECR1 gene Exon2 of polymorphic loci genetic structure analysis: the locus C, G two alleles of the CC, CG and GG 3 genotypes, eight varieties CG genotype was the dominant genotype Ningxiang pigs CC genotype detected; 3 exotic species C gene was dominant allele, five local pig breeds G gene is a dominant gene; all breeds greater genetic variation exists in the site. The Exon2 the three genotypes with meat quality traits and carcass traits related studies: the CG genotype flesh-colored level with the CC genotype flesh-colored grade significant difference (P lt; 0.05); the CC genotype marble pattern score and CG and the GG genotype marbling score differences are extremely significant (P lt; 0.01); The GG genotype fat percentage with the CC genotype fat ratio were significantly different (P lt; 0.05). 5, polymorphism loci in DECR1 gene Exon5 C of the G allele GG, CG and CC genotypes C gene was dominant allele in foreign breeds, five local pig breeds G gene for a dominant allele; seven breeds in addition Landrace CC was the dominant genotype CG genotype was the dominant genotype;, all breeds are moderate polymorphism in the locus, there is a big genetic variability. 6 Exon5 three genotypes and meat quality traits and carcass traits were the correlation analysis showed: the CG genotype pH value of the CC genotype pH value significantly different (P lt; 0.05); in the rate of water loss, GG The difference with CC genotype significantly (P lt; 0.01), CG genotype and CC genotype differences also extremely significant (P lt; 0.01) GG genotype and the CG genotype differences significantly (P lt; 0.05); GG genotype loin eye area with the CC genotype loin eye area was significantly different (P lt; 0.05); the GG genotype bone rate and CG rate of genotype bone and the CC genotype bone rate differences have reached a significant level (P lt; 0.05 ). The results of this study show that there is a correlation DECR1 the gene Exon2 and Exon5 two polymorphisms points with pig part meat quality traits and carcass traits.

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