Dissertation > Excellent graduate degree dissertation topics show

Analysis of Genetic Polymorphism of CYP2D6 in Patients with Kidney Diseases in Guizhou

Author: WenNingSui
Tutor: XieJuan
School: Zunyi Medical College,
Course: Journal of Clinical Pharmacology
Keywords: CYP2D6, gene polymorphism CYP2D6 ~ * 10 allele Kidney disease Early renal dysfunction End-stage renal failure
CLC: R96
Type: Master's thesis
Year: 2010
Downloads: 18
Quote: 0
Read: Download Dissertation

Abstract

The purpose of this project intends to Guizhou region renal insufficiency in patients with drug-metabolizing enzyme CYP2D6 gene polymorphism analysis, to explore of CYP2D6 * 10 genotype in kidney patients, the frequency distribution of the correlation with renal insufficiency. For the clinical use of drugs to individual genetic polymorphism data analysis, to provide a scientific basis for dose individualization, to improve the efficacy and reduce adverse reactions. Method for March 2009 to October 2009 in the Guizhou Provincial People's Hospital, long-term dialysis treatment, serum creatinine greater than 707μmol / L diagnosis of patients with renal insufficiency, uremia 44, and 59 serum creatinine less than 451μmol / L of early renal insufficiency patients, patients agreed extracted 2mL venous blood samples, collecting general information such as gender, ethnicity, serum creatinine, diagnosis. Extraction of DNA, using a single tube of the five primer polymerase chain reaction (PCR) amplification method CYP2D6 * 10 allele was amplified by agarose gel electrophoresis, the gel electrophoresis imager detection of CYP2D6 * 10 genotype frequency, CYP2D6 * 10 allele distribution of kidney patients in the Guizhou region meets the Hardy-Wenberg balance, whether there is a significant difference in the frequency distribution of the normal population. Results ① early renal insufficiency group CYP2D6 * 10 allele homozygote TT genotype is less than the heterozygous CT genotype also less than the wild-type homozygous CC genotype frequencies were TT genotype 0.305 the CT type 0.373, and CC-type 0.322. Frequency of allele T and C genes were 0.4915 and 0.5085. ② end-stage renal insufficiency CYP2D6 * 10 allele mutation homozygous TT genotype less than the heterozygous CT genotype and less wild-type homozygous CC genotype frequencies were TT genotype 0.296, the the CT type 0.386 and the CC type 0.318. Allele frequencies of T and C, respectively, 0.489 and 0.511. ③ early renal insufficiency and end-stage renal failure group between the genotype and allele frequencies was no significant difference in sex (P gt; 0.05). ④ CYP2D6 * 10 gene frequency per capita and CYP2D6 * 10 genotypes per capita gene frequency differences in the nephrotic early group with end-stage group no significant sex (P gt; 0.05). CYP2D6 * 10 allele frequency (5) kidney disease patients with normal population showed no significant differences. Conclusion (1) CYP2D6 * 10 gene frequency per capita and CYP2D6 * 10 genotypes per capita gene frequency in kidney disease early group and end-stage group no difference. (2) CYP2D6 * 10 genotype and allele frequencies no difference in the the nephrotic early group and end-stage group. (3) The results of CYP2D6 * 10 gene polymorphism in the occurrence of renal insufficiency, the development had no significant effect. (4) The study found that the CYP2D6 * 10 allele frequencies was 49.5%, with Chinese Miao population gene frequency of 47.15%, 51% of the Han population similar.

Analysis of Genetic Polymorphism of CYP2D6 in Patients with Kidney Diseases in Guizhou

Abstract

Related Dissertations