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The purpose of nerve growth factor NGF and its receptor p75 expression in salivary adenoid cystic carcinoma (ACC) organization, its relationship with the clinical and pathological features of the ACC and ACC perineural invasion characteristics and possible mechanisms. 2 immunohistochemical assay heparanase HPA epithelial cadherin Su E-CAD the neural cadherin N-cadherin and calcium-binding protein S100A4 in the ACC organizations and adjacent tissues, and detection of NGF antibody on human adenovirus cystic carcinoma ACC-2 cell lines in vitro intervention on the HPA, E-cad, N-cad and S100A4 expression analysis of the relationship between NGF and P75, explore the basement membrane degradation, cancer cells adhesion and movement and other aspects of the ACC addicted to the effect and mechanism of neural invasion capability. Method 1 using immunohistochemistry to detect 42 cases of ACC tissue and adjacent tissues and 5 cases of normal salivary glands of NGF, P75, HPA, E-cadherin, N-cadherin and S100A4 expression, 'and its relationship with clinicopathological The relationship between the factors. 2 Select the human adenoid cystic carcinoma cell line ACC-2 as the object of study, with a 1:20 dilution of anti-NGF antibody in vitro intervention with Immunohistochemistry was used to detect intervention before and after the HPA, E-cadherin, N-cadherin and S100A4 expression in cancer cells, and analyzed statistically. NGF positive expression rate in the ACC cancer tissues was significantly higher than in adjacent tissue expression (P lt; 0.05), higher than the P75 expression in cancer tissue expression in the cancer tissue, but the difference was not statistically significance (P gt; 0.05). NGF, P75 in ACC tumor cell cytoplasm expression; NGF expression was strong positive nerve fibers only, while the P75 was weakly positive or no expression, and perineural cancer cells staining intensity was significantly higher than that away from the nerve cancer cells. 2 N-cad, HPA, S100A4 mainly in the cell membrane and cytoplasmic expression of E-cadherin in adjacent tissue mainly in the strong membrane staining in the cytoplasm coloring mainly in cancer tissues and usually presents lightly stained or coloring. HPA, N-cad and cancerous tissue in the ACC S100A4 expression rates were 64.3%, 42.9%, 66.7%, significantly higher than the expression in the adjacent tissues (35.7%, 16.7%, 73.3%, 23.8%), significant difference (P lt; 0.05), E-cad expression in cancer tissue reduction and] HPA, N-cad, elevated expression of S100A4 and ACC perineural invasion, clinical stage (P lt; 0.05); and four with NGF (P lt; 0.05). 3 NGF and P75 expression into being related (r 0.482, p 0.001), NGF and of S100A4, N-cad, HPA's expression showed a positive related (P lt; 0.05), and of E-cad expression was negative related (r = -0.420, P lt; 0.05); the HPA and P75 expression was positively correlated (P lt; 0.05). E-cadherin and S100A4, N-cadherin and the expression of HPA was negatively correlated (P lt; 0.05); N-cadherin and S100A4, HPA expression was positively correlated (P lt; 0.05). 41:20 anti-NGF antibody in vitro intervention ACC-2 cell lines, ACC-2 cancer cells are to varying degrees expression of E-cad, N-cad, HPA and of S100A4, E-cad expression was significantly increased and N- CAD, HPA and S100A4 expression was significantly lower and showed the opposite trend. Conclusion NGF in the ACC cancer tissue was significantly higher than the adjacent tissues, and together with the P75 the PNI group was higher than NPNI group, secretion of NGF and P75 in the ACC invasive growth of cancer cells and perineural invasion process play an important role. ACC addicted neural invasion process between the nerve fibers secrete NGF receptor expressed by tumor cells P75 directed chemotaxis, NGF by regulating the expression of HPA, E-cad, N-cad, S100A4 strength and enhanced invasion ability of cancer cells, eventually leading to the occurrence of the phenomenon of the PNI. Cells intervention experiments further demonstrated that inhibition of NGF on the expression of E-cad, N-cad, HPA and S100A4 expression promoted. 3 N-cadherin, S100A4 and HPA expression in normal salivary glands, low expression in the adjacent tissues, with high expression in the ACC organization, and the two correlation prompted the development of the three in the ACC, invasion and metastasis synergy. E-cad expression of S100A4, N-cad, HPA in the ACC organization was negatively correlated, suggesting that both the occurrence and development of ACC, perineural invasion process may play an opposite effect.
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