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Objective: the use of human peripheral blood-derived dendritic cells (dendritic cells, DCs) strong antigen-presenting capacity and activation of T cells, especially naive T cells' ability to explore transfected human medullary thyroid carcinoma cell line total RNA DC vaccine induces specific cytotoxic T lymphocytes (CTL) proliferation and kill target cells efficiency. Methods: Collection of fresh normal volunteers anticoagulated peripheral blood, the application of lymphocyte separation medium (Ficoll) method with adherent mononuclear cells (PBMC), by recombinant human granulocyte - macrophage colony-stimulating factor (rhGM-CSF ), recombinant human interleukin-4 (rhIL-4), cultured in vitro for immature DC (imDC). Medullary thyroid carcinoma cell line from the extraction of total RNA was transfected DC, flow cytometry before and after transfection of DC surface CD83, CD86, CD11c, CD14 and HLA-DR phenotype variation; simultaneously by recombinant human interleukin-2 ( rhIL-2)-induced amplification of T lymphocytes, transfected DC mixed with autologous T lymphocytes stimulate tumor antigen-specific T lymphocytes obtained cytotoxic T lymphocytes (CTL), flow cytometry before and after stimulation CD3 / CD4, CD3/CD8 changes. MTT assay pulsed DC on T lymphocyte proliferation and activation of specific CTL pulsed DC on medullary thyroid carcinoma cell line transfected DC total RNA in vitro killing efficiency. Results: Extract medullary thyroid carcinoma cell line total RNA, electrophoretic display the full undegraded. Vitro human peripheral blood mononuclear cells, flow cytometry demonstrated: Not plus LPS (lipopolysaccharide, LPS) cultured DC low expression CD83, CD86, CD11c, as immature DC, in line transfected requirements; plus LPS or thyroid Total RNA medullary carcinoma cell line cultured DC, high expression of CD83, CD86, CD11c, mature DC, its typical form, have a strong ability to stimulate proliferation. Transfected DC to stimulate autologous T lymphocytes after 5 days, CD8 increase in the proportion of CTL (P lt; 0.01). Medullary thyroid carcinoma cell line total RNA pulsed DC stimulation of T lymphocytes into CTL after, MTT assay and the new transfection medullary thyroid carcinoma cell line total RNA DC cell killing effect, the results show that: with effector to target ratio increases, the killing effect was also significantly increased (P lt; 0.01). Conclusion: medullary thyroid carcinoma cell line transfected DC total RNA, may promote their maturation and effectively stimulate the proliferation of T lymphocytes with antigen-specific killing capacity of CTL, and thus play a role in its anti-cancer, medullary thyroid RNA in vivo cancer vaccine trials was provided.
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