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The Changes in Histomorphology of Mineralization Stages in Rabbits Limb Lengthening with the Technique of Celloidin Embedding Sectional Slice

Author: ZhaoZhiMing
Tutor: ShuHengSheng；ShaoZuo
School: Tianjin Medical University
Course: Surgery
Keywords: Limb Lengthening X-ray Collodion slices and Morphology
CLC: R687.35
Type: Master's thesis
Year: 2010
Downloads: 22
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Abstract

Objective: To take advantage of ordinary X-ray, collodion slices and observe and analyze the extended region of the rabbit limb lengthening callus growth on the horizontal section of new bone tissue and surrounding extend the morphological changes of the relevant organizations. Methods: 20 healthy adult Japanese white rabbits, 1/3 wire saw on the left hind leg tibial osteotomy, apply homemade single-arm fixed extension brackets preparation limb lengthening animal models, seven days after the operation began to extend, extend the rate of 1mm / d, two minutes sooner or completed, 0.5mm /. Select the mineralization of the different time points as the experimental group (mineralized section 0,7,14,28,56 days divided into five groups), were taken to extend the limb X-ray observation Limb Lengthening District callus growth and prolong limb overall force lines, and each group extended limbs drawn Preparation of collodion slices and understanding on the horizontal section and around the new bone tissue morphological changes in the process to extend the relevant organizations. Results: 1. General observation in a 10-day extension period, the extension of the limb length gradually increased, the wound dry and no significant leakage; specimens extend periosteum intact and markedly thickened and no periosteal tear traces. The 2.X line performance tibia stopped extend, extend the osteotomy gap length of about 1cm to extend the area no obvious callus image; mineralization seven days can be found in extending the area density than before the increase, a little bone in the two fracture phenomenon; visible in the first 14 days of the mineralized callus image fuzzy extend the area, and has increased over the previous 28 days; mineralization, significant increase in the amount of callus, cortical bone at the osteotomy, the medullary cavity fuzzy; mineralization in the first 56 days, extending rich regional callus, cortical and medullary cavity clear boundaries and extend the area callus healing strong, go except the mounting bracket, Jia extended limb lines of force, no malunion. Collodion slices and drawn mineralization 14 days, 28 days, 56 days, three groups of experimental animals hind legs, Preparation of collodion slices and variance analysis results show that: ① The mineralization 14 days 28 days normal tibial the bone pre osteotomy at 4 area between 56 and have a significant difference (P lt; 0.05); ② The mineralization 14 days, 28 days, the 56th day of the cross-section at the tibial nerve and tibial artery pitch no significant difference (P gt; 0.05); ③ The mineralization 14 Tian, ??28 Tian, ??the 56th day of tibial nerve and tibial bone spacing have significant forward difference (P lt; 0.05); ④ mineralization 14 Tian , 28 days, 56 days posterior tibial artery and tibial spacing significant difference (P lt; 0.05). Conclusion: Bone lengthening trauma and orthopedic surgery in recent years one of the main technical and biological principles of bone tissue regeneration process under external force pulling, this phenomenon is also known as the drafting of bone formation. Clinically, this technique has been used for limb lengthening, bone defects and infected nonunion treatment, and increased surgery, the experimental study considers: homemade single-arm external fixation the extender can successfully prepared rabbit tibial bone lengthening model; X line as a traditional inspection methods, limb lengthening, limb lines of force to extend the length, whether early detection of the axial displacement and other complications have an important role; major research methods the collodion slices and technology as sectional anatomy one can clearly show the positional relationship extend the area of ??bone and surrounding tissue structure cross-section, for an extension of the trend of the morphological changes in the structure of the process to provide a more informative reference.

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