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Objective: To investigate recombinant human IL-10 (rhIL-10) in allogeneic rabbit anti-skin allograft rejection and its impact on cytokines. Methods: 18 rabbits as a receptor, 18 rabbits were used as donor, and the establishment of an animal model, the rabbit skin grafts. Were divided into six groups: ① The group: rhIL-10 low-dose group; ② Group: rhIL-10 high-dose group; ③ The group: CsA low-dose group; ④ groups: CsA high dose group; ⑤ group: of rhIL-10 (5μg / kg) CsA (5mg/kg); ⑥ groups: saline. Observed for 28 days after the skin grafts to heal skin grafts in rabbits skin graft rejection and survival time of skin grafts; 1 day after 1 day before surgery, 4 days, 7 days, 14 days, 21 days and 28 days, blood, serum was separated, transplanted tissue IL-2, IL-4, IL-10, TNF-α and IFN-γ in the changes observed by ELISA method. Results: 1. Receptor rabbit rejection of skin grafts ①, ② 6.67 days and 9.00 days, respectively, ③, ④, ⑤ group was 12.00 days, 19.67 days and 16.16 days, ⑥ group was 4.33 days. ① group ⑥ group of relatively delayed, but no difference ②, ③, ④, ⑤ group ⑥ group compared to, rejection was significantly prolonged, and the difference was significant (p lt; 0.05). ①, ② group receptor of skin grafts in rabbits median survival time of 13.33 days and 16.00 days, respectively; ③, the ④ group to 15.33 days and 21.33 days, respectively; ⑤ group was 19.00 days; ⑥ group was 7.67 days; ① , ②, ③, ④, ⑤ group and ⑥ group respectively compared, each between the two groups were statistically significant (P lt; 0.05); ①, ② group ④ group, ⑤ group pairwise comparisons, there are also significant differences (P lt; 0.05). 3.IL-2 test results show that, the postoperative group has increased to varying degrees, which ⑥ group increased most significantly, the slow decline and reached a peak in 4-7 days, but still higher than the other groups, IL-2 increased, and the difference was significant compared with groups ②, ③, ④, ⑤ There was a significant difference (P lt; 0.05); of ① ② Group and ⑤ group. (P lt; 0.05). TNF-α test results showed that one day after surgery, ⑥ group compared with the other groups and its expression levels increased rapidly, after four days, reached a peak and then slowly decline, but still higher than the other groups, and other as compared to the respective phases, there are significant differences (P LT; 0.01), the ⑥ group and ①, ② group were compared, each have differences (P LT; 0.05); ① The group ② group compared, the difference is not significant (P gt; 0.05). 5.INF-γ test results show that, one day after surgery, ⑥ group compared with the other groups their expression levels increased rapidly after four days, reached a peak after a slow decline, but still higher than the other groups, and other each comparison, there is a significant difference (P lt; 0.01) ① The group ② group comparison, the difference is not statistically significant (P gt; 0.05). 6.IL-4 test results show that, in addition to the the ⑥ group, the expression levels of other groups have slowly increased after 4 days in each group are rising more obvious, the which the ④ group and ⑤ group, respectively, in the postoperative first 7 days peaked ①, ② group reached a peak in about 14 days, ⑥ group and other groups, a significant difference (P lt; 0.01); the ⑤ groups ①, ②, ③ were significant also are significant differences (P lt; 0.05); ① The group ② group compared the difference is not significant (P gt; 0.05). 7.IL-10 test results showed that after 1 day ⑤ group increased faster than other groups, in the first 4 days after down and has been at a low level, the other groups were different degrees, wherein ② group increased the most obvious, and reached a peak on the 7th postoperative day, ④, ⑤, ⑥ group compared with the group showed a significant difference (P lt; 0.05); ① The group ② group compare differences not significant (P gt; 0.05); the ⑤ group compared with other groups there were significant differences (P lt; 0.05). Conclusion: rhIL-10 has a role in inhibition of the rabbit skin graft rejection, delayed reaction time rejection and prolong graft survival time; rhIL-10 to reduce skin grafts in rabbits IL-2, TNF-α and INF- γ expression, indicating that rhIL-10 to achieve through the control of cytokine expression in anti-skin graft rejection effect; studies have shown that the combination of rhIL-10 and CsA reduce CsA side effects of rhIL-10 and CsA has a synergistic effect, can enhance the anti-transplant exclusion effect.
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