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Discriminant Model for the Cold and Hot Property of Traditional Chinese Medicine by HPLC-TOF MS

Author: WangYun
Tutor: XuCongJian；LiYiXue；ZhangXiaoYan；LiYanYun
School: Fudan University
Course: Obstetrics and Gynaecology
Keywords: Chinese herbal medicine Cold and heat potency Metabolomics Modernization of traditional Chinese medicine research High performance liquid chromatography - time-of-flight mass spectrometry
CLC: R285.5
Type: Master's thesis
Year: 2010
Downloads: 414
Quote: 3
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Abstract

Objective: Chinese herbal medicine theory is the core content of the traditional Chinese medicine, including cold and heat potency occupy its dominance. Traditional Chinese herbal medicine theory and more from the experience, the modernization of Chinese medicine is based in the West Pharmaceutical plant chemical and natural medicine, chemistry, under the guidance of the theory of the monomer components and less involved with TCM thinking characteristics based from The medicinal aspects systematic study. Metabolomics as the emerging discipline of systems biology, consistent way traditional Chinese medicine theory with the of metabolomics overall system. In this study, the four gas classification according to traditional Chinese medicine, select the chills and fever potency for research representatives to explore the chills and fever different herbal medicine due role in vivo metabolite changes. Based on these changes, to find changes in the relationship of potency and metabolites of the drugs cold and heat, and based on this screening candidate metabolites having the characteristic of the cold and heat potency, and to create a characteristic can be used for identification of the medicine cold and heat potency determination mode, Chinese herbal medicine determine the exact molecular theoretical basis, and can be used for the development of new drugs in the pharmacy and medicinal properties identified mindset shift has a certain role in promoting the modernization of traditional Chinese medicine research medication provides a more reliable theoretical basis for Integrative Medicine. Materials and methods: (1) in rat serum collected before and after administration of the traditional Chinese medicine: Select 19 herbs single drug particles, according to the drug four gas is divided into groups of hot, cold, cold medication group containing 10 flavored herbs, hot drug group containing 9 The taste of herbs, and the establishment of the solvent control group and blank control group. SD rats were reared under the same conditions 6 days after the row orbital venous plexus blood, serum was collected, as the control samples before the administration. Blood after administration of a single drug orally for seven days, the daily dose for human usual dose / standard weight * 20, 2ml / dose / day. Administered on day 7 two hours after the orbital venous plexus again blood, the serum was collected. . Metabolomics analysis: before and after administration of serum after addition to the pre-processing of the protein, freeze-dried, full component metabolomics analysis based on HPLC-TOF MS (liquid chromatography - time-of-flight mass spectrometry) serum. The raw data after the R statistical software, t-test and PLS-DA analysis discriminant various metabolites, the establishment of discriminant model based on traditional Chinese medicine cold and heat herbal metabolomics. T-test P value of less than 0.05 for the standard, to determine the resulting differences in metabolites compounds comparisons between different groups. The results: 1. Detected by HPLC-TOF/MS, can be effective and the system detects a change of traditional Chinese medicine for the metabolism level. 2. Detected by HPLC-TOF/MS, the detected cold and heat different medicinal drugs cause the body's metabolism to change. Hot drug metabolite 41 specific changes, cold drug metabolite 30 specific changes. By PLS-DA initially established distinction between cold and heat potency difference discriminant model, the model explained 85.3% of classified information, the model predictive capability of 61.8%. Conclusion: by high-performance liquid chromatography - time-of-flight mass spectrometry technology systems and comprehensive detection of changes in the metabolic level of Chinese medicines act on the body caused. 2 cold medicines and hot drug effects on the body are the presence of specific metabolic differences between compounds, and these differences metabolomics technology screening compounds by iconic candidate potential differences compounds. 3. Chills and fever different properties for the metabolism level change significantly, Chinese medicine cold and heat herbal discriminant model can be established by partial least squares discriminant model.

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