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The Expression and Significance of Nuclear Factor Kappa B (NF-κb) and IL-6 in the Degenerated Cervical Intervertebral Disc

Author: ZhangWenYa
Tutor: ChenHuiDe
School: Anhui Medical University,
Course: Sports Medicine
Keywords: Intervertebral disc Degeneration NF-KB IL-6
CLC: R681.55
Type: Master's thesis
Year: 2011
Downloads: 5
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Abstract


objective:Cervical intervertebral disc is a common disorder, with both genetic and nongenetic factors contributing to the development of the disease. Cervical intervertebral disc degeneration is the main pathological basis of cervical intervertebral disc. Establishing cervical intervertebral disc of rats model by unbalance of dynamic and static forces discussing the mechanism of the expression of Nuclear Factor kappa B ( NF-κB ) and IL-6 in the cervical intervertebral disc animal model by observing its image performance and the morphological changing process of Factor kappa B ( NF-κB ) and IL-6. This can offer the basis for study on the mechanism of intervertebral disc degenerationMethods: Forty SD rats no matter the sexual were enrolled and divided into two groups: The surgery group(20):The cervical muscles,and supraspinal and interspinal ligaments of rats in model group were incised through posterior approach. The skin of rats in the sham-operation group(20) was incised and then satured. Three months after operation,the establishing of model group was confirmed by X ray. The surgery group and Sham group were executed on 4 week,12weeks,after surgery to get the intervertebral disc in the surgical area after took the X-ray.The specimen were used to:(1) Conventional HE stain, morphology comparison.(2) Observing the expression of nuclear factor kappa B(NF-κB)and IL-6 by using immtmocytochemistry. Analysising the expression and the change process of the indexs by comparing the two groups to discuss the role the expression of nuclear factor kappa B(NF-κB)and IL-6 plays in the cervical intervertebral disc animel model. Results:1.Imaging findings:On 4 weeks after surgery, abnormalities on X—ray imaging can not be see in the surgery group.Degeneration on X-ray imaging was seen in the model group 3 months after operation and was more serious than in the sham group(P < 0.01).2.HE stain:Under the light microscope,interior/external layer fibrous ring showed confused and lost the normal structure in surgery group,we also observed the discontinuity and hyperplasia ofcollagenous fibers,Fibroblast in external layer fibrous ring increased;Hyperplasia of cartilage cell occurred in the nucleus pulposus next to the interior layer fibrous ring and the nucleus pulposus became thicker.But there were no the same change in Sham group.3 Immunohistochemical staining showed that the expression of NF-KB and IL-6 in the degenerated model group was higher than that in the sham operation group(P < 0.05).Conclusions : The organization of the model group were morphological and ultrastructural observations confirmed an imbalance of dynamic and static cervical disc degeneration model in rats can induce cervical disc degeneration,1 unbalance of dynamic and static forces cervical intervertebral disc animel model Can be established by using through posterior approach. Its imaging consistent with the representation reported in the clinic literature.2 nuclear factor-κB and IL -6 plays a vital role in pathogenesy, pathological change and symptom generating of cervical intervertebral disc degeneration

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CLC: > Medicine, health > Surgery > Orthopaedic Surgery ( movement system diseases,orthopedic surgery ) > Bone diseases > Spine and back disorders > The vertebrae quality hyperplasia
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