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Application of Magnetic Carriers in Immobilized Enzyme and Continuous Bioreactor

Author: JiangDaHai
Tutor: ZhaoYuanDi
School: Huazhong University of Science and Technology
Course: Biomedical Engineering
Keywords: magnetic carrier mesoporous silica material calcium alginate gel sphere enzyme immobilization continuous bioreactor
CLC: TQ033
Type: Master's thesis
Year: 2011
Downloads: 61
Quote: 0
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Abstract


In recent years, Great progress has been made on magnetic particles as immobilized enzyme carrier. Due to unique superparamagnetic properties and biocompatibility, Fe3O4 nanoparticles has become one of the most popular magnetic carriers in biotechnology. Immobilization of enzyme on magnetic carriers through physical or chemical methods has the advantage of both maintaining the high enzyme activity and facilitating recovery and recycling of enzyme in an easy and fast way.The combination of mesoporous silica materials and magnetic nanoparticles to form the magnetic mesoporous silica carrier （MMSC） could be a useful carrier due to the easily functionalized surface silanol groups and large surface area which can accommodate more target molecules. Most importantly, it can also facilitate the recovery and recycling of enzyme by applying an external magnetic field. The combination of calcium alginate gel sphere and magnetic nanoparticles to prepare magnetic calcium alginate gel （MCAG） is also studied in this paper. MCAG can embed biological macromolecules, such as enzymes or even a whole microorganism without need of chemical modification of enzymes and under mild conditions, thus rarely changes the physical, chemical and biological properties of enzymes. First magnetic nanoparticles were synthesized by co-precipitation and microwave method respectively. Magnetic nanoparticles synthesized by co-precipitation were used for MMSC preparation and that synthesized by microwave method were used for MCAG preparation. Then MMSC were modified by APTES to introduce amino groups and alkaline protease was immobilized using a coupling agent PDC. The enzyme activity results showed that the activity recovery rate was 15.1%. While the alkaline protease embedded in MCAG resulted the activity recovery rate about 54%. Finally, a high adaptable bioreactor using magnetic nano-catalysts for different processes was designed. The bioreactor has the advantages of improving the efficiency of mixing catalyst and recycling thus reducing pollution. This bioreactor provided a novel possible way for continuous using the magnetic nano-catalysts.

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CLC: > Industrial Technology > Chemical Industry > General issues > Chemical reaction process > Biochemical processes
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