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The Effects of Trichostatin A(TSA) on Proliferation and CXCR4 Expression of the Human Hepatocarcinoma SMMC-7721 Cells

Author: LuoZuo
Tutor: ZhaoQiu
School: Huazhong University of Science and Technology
Course: Internal Medicine
Keywords: hepatocarcinoma SMMC-7721 histone deacetylase inhibitor TSA Chemokine Receptor CXCR4
CLC: R735.7
Type: Master's thesis
Year: 2011
Downloads: 15
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Abstract


Objective: To explore the effects of histone deacetylase inhibitor Trichostatin A (TSA) on proliferation of the human hepatocarcinoma SMMC-7721 cells.And the effects on C hemokine Receptor 4 mRNA and protein Expression.Methods: The SMMC-7721 cells were cultured and treated with various concentration 0, 200, 400, 600 and 800 nmol/L TSA for 24h. 1. cell viability was analysed by CCK-8 assay. 2. To observe the CXCR4 protein expression in SMMC-7721 cells treated with 800nmol/L TSA by immunohistochemistry. 3. Quantitative real-time PCR, Reverse transcription-PCR assay were used to assess changes in CXCR4 mRNA expression induced by 800 nmol/L TSA. 4. Western blot analysis were used to assess changes in protein expression induced by TSA with 400 nmol/L and 800 nmol/L.Results: TSA inhibited the proliferation of SMMC-7721 cells in a dose-dependent way(P<0.05). After treated with TSA, the expression of CXCR4 mRNA and protein level up-regulation. Also increase the intensities of immunohistochemical staining.Conclusion: TSA inhibited the proliferation of SMMC-7721, but meanwile induced the expression of CXCR4 mRNA and protein level. CXCR4 was reported to mediate the metastasis and progression of variety of cancers. Therefore, the histone deacetylase inhibitor TSA can be considered as a novel therapeutic strategy for hepatocarcinoma. But use of TSA in patients as cancer therapy may paradoxically establish the potential ability of metastasis through up-regulation or reactivation of chemokine receptors CXCR4. So, we need to further investigate the mechanism of the SDF-1/CXCR4 axis in hepatocarcinoma and evaluated the effects of TSA carefully.

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