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The Preliminary Study of the Effect of Osterix on the Process of Osteoblast Differentiation from Mesenchymal Stem Cells

Author: MengLingYi
Tutor: LiuWenGuang
School: Northeast Normal University
Course: Genetics
Keywords: Osteogenic differentiation Transcription factor Runx2 Osterix HDAC TSA
CLC: R329
Type: Master's thesis
Year: 2010
Downloads: 88
Quote: 1
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Abstract


In today's society , more and more people bone disease concerns , so for the bone is more and more in-depth research . Bone is a dynamic and complex organization , in its formation and function of the process, by a variety of transcription factors, signaling pathway , the altered chromosome complexes and other factors . In which two kinds of transcription factor Runx2 and Osterix in osteoblast differentiation and bone development, bone formation process plays a decisive role , Osterix located downstream of Runx2 is Runx2 target genes . This paper focuses on the mesenchymal stem cells to differentiate into osteoblasts role during Osterix done a preliminary analysis and research. By ALP staining and von Kossa staining and RT-PCR experiments , we found that overexpression of Osterix cells , Osterix can promote mesenchymal stem cells to differentiate into osteoblasts , but its pro- differentiation does not and Runx2 strong, even with TSA , Osterix effect on osteoblast differentiation has not changed much . Followed by RT-PCR experiments , we examined the in mesenchymal stem cells to differentiate into osteoblasts in the first six days , the cells overexpressing Runx2 some osteoblast differentiation iconic expression under the action of the TSA a change such as bone sialoprotein (BSP), osteocalcin (OCN) , etc. ; while overexpressing cell lines Osterix iconic changes in gene expression levels are not as over-expression of Runx2 cell lines so dramatically , sometimes even Runx2 appearance and cell lines overexpressing the opposite phenomenon . Therefore, the role of HDAC on Osterix perhaps not so obvious that we imagine or so simple , histone deacetylase (HDAC) effects on osteoblasts may be mainly achieved by affecting Runx2 . Our results indicate that Osterix on osteoblast differentiation have a certain role in promoting , but the effect is weaker than Runx2 . In the mesenchymal stem cells to differentiate into osteoblasts , we initially explored the result is the role of HDAC on Osterix not what we want less dramatic , but also in future experiments in continuous exploration .

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