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Study on the Degradation of Steroid Compounds by Comamonas Testosteroni

Author: PanFei
Tutor: PanDaRen
School: Fujian Agriculture and Forestry University
Course: Biochemistry and Molecular Biology
Keywords: Comamonas testosteroni Soil Steroids Bioremediation
CLC: X53
Type: Master's thesis
Year: 2010
Downloads: 42
Quote: 1
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Abstract

In recent years, the problem of soil pollution increasingly aggravating, pollutants over the food chain to organisms bring serious harm to the organism. Testosterone Comamonas bacteria (Comamonas testosteroni) degradation of a variety of refractory compounds, steroidal compounds as the sole carbon and energy and consumption, has a high application value in environmental bioremediation. Many studies have focused on gene cloning and expression of a key enzyme in the bacteria in the metabolic process, as well as efficient engineered bacteria through genetic engineering techniques, but the practical application of engineering bacteria for environmental remediation rarely reported. Therefore, this study by generations of testosterone Comamonas bacteria and two strains of genetically modified (Ca3, P8) degradation stability testing, the use of various strains of steroids in the different soil degradation, simultaneous determination of total steroids and estrogen, testosterone, cholesterol content, as well as field soil degradation studies further validate the ability to degrade after genetically engineered bacteria after transformation, and to provide a theoretical basis for the engineered bacteria in environmental pollution bioremediation. The main findings are as follows: the 1 testosterone Comamonas bacteria Ct and genetically modified bacteria P8 Ca3 subculture 10, 20, 30, 40, 50 on behalf of, the determination of the degradation of different strains of different concentrations of testosterone. The results showed that the ultimate degradation rate differences between the various strains to reach a significant level, the degradation rate of P8, 46%; most suitable for the degradation of testosterone concentration 100μg/mL; generations degradation rate difference was not significant, minimum degradation subculture bacteria rate accounted for 78% of the original bacteria, the wild-type strain engineered bacteria degradation stability. ⑵ Ct and genetically modified bacteria of the wild-type strain P8, Ca3 steroidal compounds in soil degradation, the results show that there are significant differences in the level of each factor on various soils, strain, degradation time. Different types of soil, the degradation rate of the P8 maximum of 38%, 8.9 percentage points higher than Ct; different vegetation, soil, the Ca3 the degradation rate of up to 22.6%, 3.5 percentage points higher than Ct; different underwater sludge , P8 degradation rate up to 40.5%; different locations in the the lotus pool bottom mud P8 degradation rate up to 31.6%. The rate of degradation of each strain is gradually increased as the degradation time prolonged. The steroidal compound concentrations in the different soil samples vary, leading to the degree of degradation of the different strains of its different steroidal compounds concentration level of testosterone degradation of Aeromonas influential. (3) by measuring testicular ketone Cong wool single-cell bacteria on the soil estrogen hormone, testosterone, cholesterol degradation of the situation, the results show that, genetic engineering in rice soil bacteria P8, between Ca3 the degradation rate with wild strains Ct achieve significantly with the level, P8 the degradation rate of 29.3%, and 24.8% for Ct strongest degradation estrogen; Matilda House, in the lotus pool bottom mud, the degradation rate of the genetically engineered bacteria and wild mushroom also reached a significant level, P8 the degradation rate of 30.5%, while the Ct of 24%, the strongest degradation of testosterone. (4) through the field soil degradation test, the results also show that the genetically modified strain P8 Ca3 degradation effect than the wild-type strain Ct. Put the engineered bacteria and put in engineered bacteria and chicken manure into the soil degradation effects of the latter than the former, and achieve a significant difference. Therefore, application of chicken manure organic fertilizer in the soil, the engineered bacteria significantly improve the rate of degradation of steroidal compounds. The above findings testosterone Comamonas bacteria in the actual bioremediation applications provide a theoretical basis, Comamonas testosteroni using rational development and utilization of genetically engineered bacteria.

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CLC: > Environmental science, safety science > Environmental pollution and its prevention > Soil Contamination and Control
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