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Fraction and Preparation of Extracellular Metabolites from Liquid Culture Agrocybe Chaxingu Huang and Their Antioxidant Characters

Author: LinDaRong
Tutor: ShenHengSheng
School: Fujian Agriculture and Forestry University
Course: Animal Nutrition and Feed Science
Keywords: Agrocybe chaxing Camellia branch Wheat bran Liquid culture Vitro and in vivo antioxidant
CLC: S816
Type: Master's thesis
Year: 2010
Downloads: 64
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Abstract


Extracellular metabolites in vitro and in vivo antioxidant properties of liquid culture in order to study the Agrocybe chaxing (Agrocybe chaxingu Huang), this study Camellia branch with wheat bran as the main raw material, through three trials: comparison of different nutritional matrix Agrocybe chaxing liquid culture cells polysaccharides and phenolics content and in vitro antioxidant properties and research not compatriots outside metabolite separation of liquid culture preparation techniques (test); using β-cyclodextrin liquid outer metabolites culture supernatant liquid analysis the Agrocybe chaxing extracellular metabolite mixture on the organs of the mice with the index and serum antioxidant indicators (test); based on further analysis the Agrocybe chaxing extracellular polysaccharides and phenolic metabolites of small rat growth performance, organ index and serum antioxidant indicators (test). Wheat bran, Camellia sticks water extract of the medium as the control (CK-WB and CK-YC) extract medium (WB-YC) for comparison with wheat bran mixed water and Camellia sticks; measured liquid culture tea paid mushrooms in the supernatant polysaccharide and phenolic content and in vitro antioxidant properties; extracellular polysaccharides and phenolic product freeze-drying alcohol chromatographic separation and analysis of the polysaccharide and polyphenol content of the product was prepared. Agrocybe chaxing liquid culture: supernatant, extracellular polysaccharide content CK-the WB group with WB-YC group was higher than CK-YC group (P lt; 0.05), and the content of phenolic metabolites WB-YC group and CK-WB group was higher than CK-YC group (P lt; 0.05); The three groups Agrocybe chaxing liquid culture supernatant of hydroxyl radicals in vitro clearance order: CK-WB gt; the WB-YC gt; CK-YC (P lt; 0.05). Β-cyclodextrin (β-CD)-coated, spray-drying the mixed powder prepared A. chaxingu extracellular outer metabolites; with 4% Camellia sticks water extract group (CK-YC) as the control mice were different gavage level of the comparative experiment The results showed: intragastric administration of different doses of 4% water extract of Camellia sticks (CK-YC) culture supernatant preparation the Agrocybe chaxing liquid and 3% water extract of wheat bran 4% Camellia branch water extract (WB-YC) extracellular metabolites, thymus index was lower than the saline gavage group was to improve the trend; to gavage 600-800mg/kg · bw · d dose WB-YC Group spray dried product was prepared in serum SOD indicators significantly higher than the control group (P lt; 0.05). Alcohol chromatographic separation, freeze-dried preparation the Agrocybe chaxing liquid culture exopolysaccharides and extracellular phenolics; analysis of mice administered test in vivo antioxidant characteristics. The test results show that: Compared with the control group, the the WB-YC group with the group of CK-YC the Agrocybe chaxing extracellular phenolic metabolites can significantly increase serum SOD activity (P lt; 0.05), while the other group Agrocybe chaxing liquid culture exopolysaccharide also show the role of SOD activity in the blood of mice. At the same time, the blood of mice in MDA content analysis also showed that, compared with the control group, 3% wheat bran 4% Camellia sticks polysaccharide group (P lt; 0.05), of each Agrocybe chaxing extracellular metabolite gavage group are different minimize the MDA content in the blood. Moreover, respectively the gavage Agrocybe chaxing liquid culture the exopolysaccharide extracellular phenols, no significant effect on the role of the organs of the mice Index.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > General Animal Science > Feed
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