|
Catalpa tree (Catalpabungei). Bignoniaceae Catalpa genus of deciduous trees, native to China. Catalpa tree is a traditional Chinese cultivation of valuable timber species, and the famous ornamental tree, the ancients reputation to the wood King laudatory. Catalpa wood physical properties, chemical stability, and are widely used in high-quality furniture, appliances and shipbuilding, military, and other special materials. Catalpa tree tree tall, deep roots, applied to farmland shelter forest, can significantly improve the protection of the region. As tree species, the catalpa tree can be used for the greening of the residential areas, improve the vegetation cover. With hysteresis dust, absorb harmful gases, and many other functions, in particular, there is strong resistance to the toxic gases such as sulfur dioxide, chlorine, can significantly improve the ecological environment in the urban areas. In this study, round base long fruit Catalpa stem segments for the multiple shoots Iduced, rapid propagation study with clustered buds ways regenerated plants. Focused on the selection and sterilization of explant profusion bud induction, shoot proliferation, rooting, plantlets out such as bottles Lianmiao transplanted a few focused on the selection of different sterilization methods and multiple shoots induced profusion bud proliferation, rooting of various stages, such as to optimize the original medium, the choice of additive and its amount of different exogenous, agar concentration, as well as selection of anti-oxidant, and many other related problems. Medium in the test program using orthogonal design, the the interspersed single factor compared general design method. Visual analysis of data using SPSS software analysis and comparison of single factor data. This document is intended by the above test, a comprehensive study of the round base long fruit Catalpa tissue culture of the various stages of exogenous influence factors and optimization of medium formula, filter out the best combination of technical parameters, round base long fruit Catalpa optimal regeneration system for Catalpa the tree excellent germplasm resources preservation and excellent promotion the foundation, also round based long fruit Catalpa standardization, industrialization, large-scale nursery to provide technical support, and solve the problem of market catalpa tree shortage of fine varieties of seeds and seedlings. The results show that: 1. The round base long fruit Catalpa explants sterilization method is tap water: the round base long fruit Catalpa stem section → washing powder supernatant soak for 30 minutes with a soft brush to remove surface dirt → 30min → 1.25g · L -1 30 minutes carbendazim dip hyperstatic stage 8S → 70% alcohol soaked sterile water rinse → 2 → 0.1% mercuric chloride sterile water rinse Baptist 10min → 10 → autoclaved 100mg · L -1 PVP soak for five minutes → inoculated in MS 6BA0.8 mg · L -1 IBA0.1mg · L - 1 30mg · L -1 Penicillin 30mg · L -1 streptomycin 1g · L -1 activated carbon medium a success rate of 16.67%. Also found that the interception of about 0.5 ~ 1 cm stem segments as explants more appropriate, easy to sprout, light pollution, less browning in the bud bar, sunny afternoon plus trees in semi-lignified between March and April. During the early experimental culture medium supplemented with 0.5 mg · L -1 1 Shannong, also, can significantly reduce the contamination rate and does not inhibit the germination of axillary buds. Impact circular base long fruit Catalpa axillary buds exogenous additives: penicillin and activated carbon. The most suitable medium for exogenous additives penicillin 30 mg · L -1 activated carbon 1.0 g · L -1 PVP100mg L -1 . 3. Impact circular base long fruit Catalpa clustered buds proliferation exogenous additives paclobutrazol, the optimum dosage of 0.2mg · L -1 . Also found that the processing in a medium appropriate to add some antioxidants, such as VC, can be effectively weakened browning, without adding agar, proliferative effect will be better. 4. Rooting stage exogenous additives in addition to the activated carbon can be significantly inhibited by addition to the the new rooting growth and elongation, other exogenous additives on new rooting growth, elongation, the increase did not significantly affect, but also found in 5 g · L -1 under the conditions of the presence of activated carbon, add 0.8mg · L -1 of IBA higher rooting rate, root longer description of activated carbon and IBA all play a role. Liquid culture and solid culture of rooting rate difference is not too large, the largest number of treated plantlets rooting liquid medium, 22.43 and root length longer. 5 additional test tube transplanting of rooted plants 1/2MS the mother liquor, will significantly improve the survival rate of transplant. And rooting techniques explored the round base long fruit Catalpa bottle: between mid-May to June, the circular base long fruit Catalpa tube seedlings removed from the culture medium, clipping high as 2.5 to 3.0 the young rootless cm tissue culture, 2 to 3 leaves reserved. 150 mg · L -1 concentrations of 2,4-D for 24h, and then 0.3% potassium permanganate dip 30min, filled with cuttings in perlite matrix nutritional bowl final. Nutrient solution mix thoroughly with 1/2MS, after the end of the coating moisturizing every morning and evening Jiemo 1 ~ 2min for ventilation, and at a later time in accordance with the seedlings need water the degree of discretion foliar spray with water to increase humidity.
|