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The present study was to utilize Practice 八号 \cytological effects of alfalfa, physiological and biochemical indicators of seedling growth potential and the detection of RAPD molecular techniques for space mutation breeding of alfalfa to provide experimental basis. The results of the study are as follows: \, an increase of 20.27%, 19.53%, 18.54%, 12.73%, 23.70%, 27.03%, 21.85%, 12.28%, respectively. And the eight varieties alfalfa chromosome occurrence of different types of distortion, distortion rates were 5.12 ‰, 9.76 ‰, 9.78 ‰, 15.26 ‰, 14.36 ‰, 4.45 ‰, 3.73 ‰, 7.99 ‰ to the largest proportion of chromosome fragments. WL232 No. 1 WL323HQ, BeZa87 Pleven6 801 of Longmu, the Longmu 803, Zhaodong, grasslands 8 alfalfa leaf peroxidase (POD) activity, soluble protein content, superoxide dismutase (SOD was determined ) activity. Practice 8 \the soluble protein content of alfalfa varieties were increased, respectively, increased by 8.02%, 6.00%, 15.64%, 14.63%, 17.12%, 3.44%, 13.64%, 7.68%; eight varieties of alfalfa leaves SOD activity decreased, respectively, to reduce 7.39%, 2.78%, 11.94%, 6.42%, 6.34%, 4.03%, 4.81% and 12.47%. 8 alfalfa peroxidase (POD) activity and soluble protein content as well as reduce the activity of superoxide dismutase (SOD) and resistance to adversity inflicting injury. Determination of the eight varieties of alfalfa seedlings root length, germination percentage, fresh weight. The results show that the Shijian - 8 \animal husbandry, 801, Longmu 803 Pleven6 BeZa87 and WL323HQ germination rate is lower than that of the control. Zhaodong, 801 of Longmu, Longmu 803 Pleven6 and WL232 plant height compared to the control reduced 7.84%, 2.94%, 0.88%, 4.06%, 6.10%, grassland No. 1, BeZa87 and WL323HQ of plant height compared to the control respectively, an increase of 8.78%, 8.47%, 2.69%; the Zhaodong Longmu 801, the Longmu 803, Pleven6 the, BeZa87 WL232 and WL323HQ fresh weight compared to the control decreased by 6.52%, 8.70%, 2.27%, 6.00%, 6.82%, 14.29%, 12.27%, compared to the prairie No. 1 fresh weight was increased by 8.33%. RAPD random amplified using 36 primer Practice 8 \good and highly polymorphic bands. Polymorphisms, including new and missing bands. SPACE FLIGHT WL232 No. 1 WL323HQ, BeZa87 Pleven6 801 of Longmu, the Longmu 803, Zhaodong, grassland eight varieties of alfalfa SP1 generation RAPD polymorphic rate were 18.06%, 14.47%, 7.04%, 13.70%, 15.00%, 14.29? .23% and 11.39%. Including new bars with polymorphic highest rate is WL232 varieties was 9.72%; while the lack of bands polymorphic highest rate Zhaodong varieties missing bands polymorphism was 12.82%, the Zhaodong variety of polymorphic the highest rate of varieties.
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