Dissertation > Excellent graduate degree dissertation topics show

Studies on Tissue Culture and Plant Regeneration of Perennial Chamomile

Author: GaoLinNa
Tutor: JiangChangYang
School: Liaoning Normal University
Course: Botany
Keywords: Perennial chamomile Tissue Culture Rapid Propagation clone Regeneration
CLC: S567.239
Type: Master's thesis
Year: 2010
Downloads: 100
Quote: 3
Read: Download Dissertation

Abstract


The study was mainly to probe an efficient way through tissues culture and rapid propagation of Perennial chamomile. The stems with axillary buds and the stems without axillary buds were both taken as explants. The factors that affect tissue culture including basic medium and hormones were detailed investigated. As a result, the optimum mediums of tissue culture were screened. A plant regeneration system of Perennial chamomile was estabilished in this research, which provide the theoretical base and technical measures for protection and industrial producing of this wildlife resource. The main results as follows:1. Callus induction of aseptic stems: The aseptic stems withoout axillary buds in vitro can be used for inducing callus, and the optimum medium was MS+BA1.0mg·L-1+NAA0.1 mg·L-1. The initial rate was 90%.2. Proliferating cultivation of callus: The optimum culture medium for proliferating callus was MS+BA1.0 mg·L-1+NAA0.1 mg·L-1, proliferating rate was 93.33%.3. Callus differatiation: The optimum culture medium for differentiating callus was MS+BA1.0 mg·L-1+NAA0.5 mg·L-1, differentiating rate was 82%.4. Induction of axillary buds of stems: The stems with axillary buds of young branches were taken as explants. The optimum culture medium for axillary buds’induction was MS+BA0.5 mg·L-1+NAA0.2 mg·L-1, the induction rate was 92%, bud number was 5.4.5. Rooting culture:The optimum culture medium for rooting was 1/2MS+IAA0.2 mg·L-1. The rooting rate could be up to 100%, the root number was 4.9.6. Proliferating cultivation of roots: The optimum culture medium for proliferating roots was 1/2MS+ IAA0.2 mg·L-1, the root number was 4.93. Under the conditions of variable temperature and in the lights, the optimum medium for rooting was 1/2MS + IAA0.2 mg·L-1, The rooting rate was 100%. Under the conditions of constant temperature and in the lights, 1/2MS + IAA0.2 mg·L-1and 1/3 MS + IAA0.2 mg·L-1 were the optimum mediums for rooting, the rooting rate were both 86%. Under the conditions of constant temperature and in the dark, the optimum medium for rooting was White + IAA0.2 mg·L-1, the rooting rate was 73%. Under the conditions of variable temperature and in the dark, the optimum medium for rooting was White+ IAA0.2 mg·L-1, the rooting rate was 87%. 7. Transplanting and cutting:The optimmu transplanting and cutting medium forperennial chamomile is cinder,in which the survival rate was very high and growing very well.

Related Dissertations

  1. The Ah Chengarchitectural Regeneration Design Research Concerned about the Jin Dynasty Culture,TU984.114
  2. Cloning and Expression Analysis of GPx, GST and SAHH Genes in Chlamydomonas Sp. ICE-L from Antarctica,Q943.2
  3. Effects of Light Control on Tissue Culture and Photosynthesis Characterristics of Anthurium Andraeanum Lind,S682.14
  4. Cloning and Characterization of BAFF Gene from Whitespotted Catshark and Crucian Crap,S917.4
  5. Study on Rapid Propagation of Energy Plant Euphorbia Tirucalli,Q949.93
  6. Ion Exchange Removal of Hexavalent Selenium in Raw Water,X703
  7. Hani settlement vernacular architecture of renewable road,C95
  8. Study on the Key Techniche of Tissue Culture of New Phalaenopis ’EVER Greatduble Dragon,S682.31
  9. Research on Influnce of Silicon and Sulfur on Arsenic Uptake and Accumulation of Rice (Oryza Sativa L.),S511
  10. Cloning Andanalysis of NAC Transcription Factor Gene in Tobacco(Nicotiana Tabacum L.),Q943.2
  11. Cloning and Molecular Analysis of Lovastatin Biosynthesis Related Genes from Monascus Purpureus,TQ927
  12. Evaluation on Forage Quality and Biomass Energy Characters of Inbred Vegetative Lines of Napier Grass,S543.9
  13. Construction of Expression Vector and Genetic Transformation in Phalaenopsis of Phalaenopsis Floral Meristem Identity Gene LFY,S682.31
  14. Studies on the Mechanism and Recovery Measures of Hyperhydricity of Pyrus Calleryana Decne in Vitro,S661.2
  15. Histological Studies of Leaf Regeneration and Fact Ors Influencing the Efficiency Agrobacterium Mediated Transformation in Zizyphus Jujube. Mill.,S665.1
  16. Study on Key Technology and Its Physiological Basis of Virus Elimination and Rapid Propagation of Phalaenopsis,S682.31
  17. Development of Energy-efficient Cultivation System and the Practical Application in Ornamental Plant Tissue Culture,S688
  18. Preliminary Study on the Regeneration System and the Reporter Gene GFP Instantaneous Expression of Soft-seed Pomegranate Variety ’Tunisia’,S665.4
  19. Research on the Efficiency of Circle-Flow Constructed Wetland Process for Swine Wastewater Treatment and Zeolite Regeneration,X713
  20. Study on Preservation Method and Strains Variation of Soybean Mosaic Virus in Soybean Production Areas in Anhui Province,S435.651
  21. Study on the Tissue Culture and Seed Germination Rate Modelling of Highbush Blueberry,S663.9

CLC: > Agricultural Sciences > Crop > Economic crops > Medicinal crops > Herbal > Perennial > Other
© 2012 www.DissertationTopic.Net  Mobile