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Transformation of ppdk of Maize to Indica Rice and the Effect on Photosynthetic Traits

Author: ZhangYiHua
Tutor: HeShuiLin；XieHuaAn；ZhangJianFu
School: Fujian Agriculture and Forestry University
Course: Biochemistry and Molecular Biology
Keywords: Corn Pyruvate phosphate dikinase PPDK Transgenic rice Agrobacterium
CLC: S511.21
Type: Master's thesis
Year: 2010
Downloads: 29
Quote: 0
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Abstract


Rice belongs to C3 plants, the lower limit of its biological photosynthetic efficiency become a major production to further improve internal factors. In recent years, large panicle rice breeding in the direction towards the development of its \Because C4 plants with high photosynthetic efficiency, CO2 compensation point is low, light and low breathing, especially in bright light, heat, drought and other adverse conditions, C4 plants have significant growth advantage and water and nutrient use efficiency, biological yield is higher. Thus, the transfer of C4 plants in rice photosynthetic characteristics for improving its \Dual pyrophosphate pyruvate kinase (PPDK) is a C4 plant photosynthetic pathway is one of the key enzymes that catalyzes the formation of the C4 photosynthetic pathway PEP is another key enzyme phosphoenolpyruvate carboxylase (PEPC) substrate for CO2 fixation In C3 plants to improve its expression may be one of the ways the relationship between source and sink. Indica restorer line \In this study, maize own promoter containing the full ppdk Ti plasmid gene expression vector plants ultra pC1380-ppdk introduced into Agrobacterium, the \Photosynthetic Physiological analysis was carried out to obtain the following results: 1. by Agrobacterium-mediated method vector pC-1380-ppdk Import \resistant callus for differentiation from five independent resistant callus obtained 13 resistant plants, the conversion was 16.8%, green plant regeneration rate was 10.9%. 2. PCR and dot blot results showed that maize ppdk gene has been initially integrated into indica restorer line \3 pairs of transfected genes in maize ppdk indica restorer line \), water use efficiency (WUE) were increased up 44.98%, 26.53%, was significant. The results of this study in order to further explore the ppdk C3 plant source-sink relationships in the value of genetic improvement and screening of source-sink relations coordination indica restorer line \

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CLC: > Agricultural Sciences > Crop > Cereal crops > Rice > Meters of sticky points > Indica
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